现代医药卫生2016，Vol.32Issue(7)：961-965,5.DOI:10.3969/j.issn.1009-5519.2016.07.001
维拉帕米治疗高脂血症胰腺炎机制研究
Research on mechanism of verapamil in treating hyperlipidemic pancreatitis
摘要
Abstract
Objective To explore the mechanism of verapamil intreating hyperlipidemic pancreatitis (HLP ). Methods Eighty SD rats were randomly divided into the sham operation group (SO),severe acute pancreatitis group (SAP),HLP model group and verapamil treatment group(CCB),20 cases in each group. The mortality rate was calculated after 24 h, then rats were killed for taking pancreatic tissue and collecting blood by puncture. The blood triglyceride (TG) level was detected. The levels of IP3 in pancreatic tissue,serum PFA and TXA2 were detected by ELISA. The flow cytometry was used to measure pancreatic intracellular calcium ion level. The acid titration method was used to measure serum PLA2. The pancreatic histological injury was evaluated by HE staining. Results (1)The 24 h mortality rate:the mortality rate in the SO group was lower than that in the other three groups with statistical difference(P<0.05). The 24 h mortality rate in the HLP group was higher than that in the SAP and CCB groups with statistical difference(P<0.05).(2) The TG level:the TG level in the HLP and CCB groups was significantly increased compared with the SAP and SO groups with statistical difference(P<0.05).(3)The IP3 level in pancreatic tissue:the IP3 level in pancreatic tissue had statistical differences between the SO and SAP groups,between the SAP and HLP groups and HLP and CCB groups(P<0.05). (4)The calcium ion level in pancreatic acinar cell:the calcium ion level in the SAP group was higher than that in the SO group , the calcium ion level in the HLP group was higher than that in the SAP group ,while the calcium ion level in the CCB group was lower than that in the HLP group with statistical difference (P<0.05). (5)The serum PLA2 level:the serum PLA2 level in the SAP group was significantly higher than that in the SO group ,whereas serum PLA2 level in the HLP group was significantly increased compared with the SAP and CCB groups with statistical difference(P<0.05).(6)The serum PAF and TXA2 levels:the serum PAF and TXA2 levels had statistical differences between the SO and SAP groups (P<0.05);the serum PAF and TXA2 levels in the HLP group was significantly increased compared with the SAP and CCB groups with statistical difference (P<0.05). (7)The pancreatic histological scores:the injury in the SAP group was significantly increased compared with the SO group with statistical difference (P<0.05);the pancreatic injuries score in the HLP group was significantly increased compared with the SAP and CCB groups with the statistical differences(P<0.05). Conclusion Calcium overloading plays an important role in the development of HLP rat models. Verapamil has remarkable effect in the treatment of HLP rat models.关键词
高脂血症/胰腺炎/磷脂酶A2/维拉帕米/钙/代谢/大鼠,Sprague-Dawley/死亡率Key words
Hyperlipidemias/Pancreatitis/Phospholipases A2/Verapamil/Ca/metabolism/Rats,Sprague-Dawley/Mortality引用本文复制引用
彭兰,李文科,吴礼国,黄晓丽,王东..维拉帕米治疗高脂血症胰腺炎机制研究[J].现代医药卫生,2016,32(7):961-965,5.基金项目
四川省科技厅项目基金资助（2012FZ0033）。 （）
