现代医药卫生2016，Vol.32Issue(7)：966-968,3.DOI:10.3969/j.issn.1009-5519.2016.07.002
4′，5，7-三羟基异黄酮对LPS诱导的人主动脉内皮细胞ephrinB2及IL-6 mRNA表达的影响
Effects of 4′,5,7 trihydroxyisoflavone on mRNA expression of ephrinB2 and IL-6 induced by LPS in human aortic en-dothelial cells
摘要
Abstract
Objective To study the effects of 4′,5,7 trihydroxyisoflavone on mRNA expression of ephrinB2 and IL-6 in duced by LPS in human aortic endothelial cells (HAEC). Methods Human aortic endothelial cells were cultured in vitro and 0.1,1.0,10.0,100.0,1000.0μg/mL of LPS was to stimulate HAEC for 4 h. The HAEC injury model was established. Then the cultured HAEC were divided into the model group(0.1μg/mL LPS),low,middle and high 4′,5,7 trihydroxyisoflavone concentra tion groups(1.0,10.0,100.0μm/mL respectively);the cell vitality was detected by MTT assay. The mRNA expressions of ephrinB2 and IL-6 were detected by reverse transcription polymerase chain reaction (RT-PCR). Results Except for the 10.0,100.0μg/mL LPS groups,the rest other concentration groups all could reduce the vitality of HAEC compared with the control group ,the differ-ences were statistically significant(P<0.05),LPS could promote the mRNA expression of ephrinB2 and IL-6;the middle and high concentration 4′,5,7 trihydroxyisoflavone groups HAEC could promote the increase of HAEC vitality with statistical difference (P<0.05) and the low and middle concentration 4′,5,7 trihydroxyisoflavone groups could significantly reduce the mRNA expres-sion of ephrinB2 and IL-6 with statistical difference(P<0.01). Conclusion Appropriate concentration of 4′,5,7 trihdroxy-isoflavone has a certain protective effect to LPS induced HAEC injury.关键词
异黄酮类/内皮细胞/白细胞介素6/脂多糖类/细胞增殖/炎症/ephrinB2Key words
Isoflavones/Endothelial cells/Interleukin-6/Lipopolysaccharides/Cell proliferation/Inflamma-tion/ephrinB2引用本文复制引用
姜念,周雪冰,欧刚卫,陆红玲..4′，5，7-三羟基异黄酮对LPS诱导的人主动脉内皮细胞ephrinB2及IL-6 mRNA表达的影响[J].现代医药卫生,2016,32(7):966-968,3.基金项目
贵州省科技厅联合基金重点项目（黔科合J字LKZ[2013]14）。 （）
