烟草科技2016，Vol.49Issue(4)：1-7,7.DOI:10.16135/j.issn1002-0861.20160401
基于高通量测序的废次烟末水提液中细菌群落分析
High-throughput bacterial analysis on aqueous extract of waste tobacco scrap
摘要
Abstract
To investigate the variation of microorganisms in aqueous extract of waste tobacco scrap during paper-making process of reconstituted tobacco sheet materials, Roche 454 FLX pyrosequencing was adopted to analyze the diversity of 16S rRNA gene sequences of bacteria in the aqueous extract of waste tobacco scrap. The variation of bacterial communities and their abundances in the extracts was obtained. The results showed that: 1) The aqueous extracts of waste tobacco scrap, including the original extract (M1), the concentrated extract (M2) and the aged extract (M3), harbored abundant levels of bacteria. The dominant phyla was Firmicutes. Lactobacillus and Lysinibacillus were the dominant genera of Firmicutes in all the samples. 2)It was also found that the abundance of Lactobacillus decreased from M1 to M3, while the abundance of Lysinibacillus increased. The variation of bacterial community based on high-throughout sequencing provides a scientific basis for improving the quality of paper-making reconstituted tobacco materials.关键词
造纸法再造烟叶/废次烟末水提液/16SrRNA/454FLX焦磷酸高通量测序/细菌群落Key words
Paper-making reconstituted tobacco material/Aqueous extract of waste tobacco scrap/16S rRNA gene/454 FLX pyrosequencing/Bacterial community分类
轻工纺织引用本文复制引用
舒明,樊虎,刘金莉,杨洋,许式强,高阳,周国俊..基于高通量测序的废次烟末水提液中细菌群落分析[J].烟草科技,2016,49(4):1-7,7.基金项目
浙江中烟工业有限责任公司重点科技项目“基于造纸法再造烟叶品质调控的生化技术研究与应用”（ZJZY2013C002）。 （）
