疑难病杂志2016，Vol.15Issue(5)：514-517,4.DOI:10.3969/j.issn.1671-6450.2016.05.020
右美托咪定预处理对大鼠缺血再灌注心律失常的影响及机制研究
Efects and mechanisms of dexmedetomidine preconditioning on ischemia-reperfusion arrhythmia in rats
李霞 1刘芬 1董航 1江群 1熊海霞1
作者信息
- 1. 430000 武汉，江汉大学附属医院麻醉科
- 折叠
摘要
Abstract
Objective To investigate effects and mechanisms of dexmedetomidine preconditioning on ischemia-reper-fusion arrhythmia in rats.Methods From March to June 2015 in Basic Mesical College of Wuhan University,30 rats were di-vided into sham operation group ( SO group) , ischemia-reperfusion group ( I/R group) and dexmedetomidine preconditioning group ( DEX group) ,10 rats each group.Firstly, the coronary artery was ligated for 30 min to cause myocardial ischemia, and then the ligature was loosened for 120 min to give the myocardium reperfusion.Lines were given but not ligated in SO group, 100 μg/kg Dexmedetomidine was given intra-peritoneal at 2h before ischemia in DEX group, and the same volume physiologi-cal saline was given in I/R group.The type and the scores of arrhythmia were monitored and recorded in each group.The mR-NA level of Cav1.2 and NCX were detected by the real-time PCR.Results There was no significant changes of cardiac rhythm in SO group, and the score of arrhythmia was 0.The incidence of arrhythmia were lower in DEX group than in I/R group ( P <0.05), and the scores of arrhythmia within 30 min of ischemia and 120 min of reperfusion in DEX group were both lower than in I/R group ( P <0.05).Compared with SO group, the mRNA levels of Cav1.2 and NCX increased in both I/R group and DEX group ( P <0.05), and which were significantly lower in DEX group than in I/R group.Conclusion Dexmedetomidine preconditioning can reduce the incidence of arrhythmia, which is related to the mechanism that dexmedetomi-dine reduces Cav1.2 and NCX mRNA levels to inhibit calcium overload.关键词
右美托咪定/预处理/缺血再灌注损伤/心律失常/大鼠Key words
Dexmedetomidine/Preconditioning/Ischemia-reperfusion injury/Arrhythmia/Rats引用本文复制引用
李霞,刘芬,董航,江群,熊海霞..右美托咪定预处理对大鼠缺血再灌注心律失常的影响及机制研究[J].疑难病杂志,2016,15(5):514-517,4.
