医药导报2016，Vol.35Issue(5)：448-453,6.DOI:10.3870/j.issn.1004-0781.2016.05.004
石菖蒲-远志药对不同组分对阿尔茨海默病模型大鼠海马 Tau 蛋白及其 Ser396位点磷酸化的影响
Effect of Different Components of Acorus tatarinowii and Polygala tenuifolia on p-Ser396 and Tau-5 Expression in the Hippocampus of Rats with Alzheimer′s Disease
摘要
Abstract
Objective To investigate the different components of Acorus tatarinowii and Polygala tenuifolia(volatile oil, aqueous extract)on the expression of phosphorylated Tau protein at site Ser396 and Tau-5 in the hippocampus of rats with Alzheimer′s disease(AD). Methods Male Sprague Dawley rats were randomly divided into 8 groups:normal control group, model control group,low-,middle-,and high-dose groups of volatile oil of Acorus tatarinowii and Polygala tenuifolia,and low-, middle-,and high-dose groups of aqueous extract of Acorus tatarinowii and Polygala tenuifolia. The subacute aging model was established by intraperitoneal injection of D-galactose( D-gal). Rats were given different components of Acorus tatarinowii and Polygala tenuifolia(crude drug dosage,0.6,1.2,1.8 g·kg-1 )in experimental groups,and 0.9% sodium chloride solution in normal control group and model control group,by gavage for 28 days.The levels of phosphorylated Tau protein at site Ser396 and Tau-5 were detected in hippocampal tissues by Western blotting and immunohistochemistry. Results The levels of phosphorylated Tau protein at site Ser396 were significantly enhanced in the model control group,as compared with those in normal control group (P<0.01).The relative expression levels of Tau protein Ser396 were 3.83±0.10,3.35±0.01,3.11±0.01,2.75±0.03,2.93±0.01,2.55± 0.07,and 2.23±0.08 in model control group,low-,middle-,and high-dose groups of volatile oil of Acorus tatarinowii and Polygala tenuifolia,and low-,middle-,high-dose groups of aqueous extract of Acorus tatarinowii and Polygala tenuifolia,respectively.Two components of Acorus tatarinowii and Polygala tenuifolia could dephosphorylate Tau protein Ser396 to vary degrees in a dose-dependent manner.The aqueous extract component was slightly better than the volatile oil component,especially in the high-dose group(P<0.01).But Tau-5 did not change significantly after treatment with Acorus tatarinowii and Polygala tenuifolia(P>0.05). Conclusion Acorus tatarinowii and Polygala tenuifolia could promote the dephosphorylation of Ser396 site of Tau protein in the hippocampus of AD rats,with the aqueous extract component having better effects.关键词
石菖蒲/远志/挥发油/水提物/阿尔茨海默病/海马/Tau 蛋白Key words
Acorus tatarinowii/Polygala tenuifolia/Volatile oil/Aqueous extract/Alzheimer′s disease/Hippocampus/Protein Tau分类
医药卫生引用本文复制引用
张智华,王秀莲,吕银娟,柳琳,吴建红..石菖蒲-远志药对不同组分对阿尔茨海默病模型大鼠海马 Tau 蛋白及其 Ser396位点磷酸化的影响[J].医药导报,2016,35(5):448-453,6.基金项目
国家自然科学基金资助项目 （）
