医药导报2016，Vol.35Issue(5)：454-457,4.DOI:10.3870/j.issn.1004-0781.2016.05.005
补脑胶囊对阿尔茨海默病模型大鼠学习记忆和抗氧化能力的影响
Effect of Bunao Capsule on Learning,Memory and Antioxidative Abilities of Rats with Alzheimer′s Disease
摘要
Abstract
Objective To investigate the effect of Bunao capsule on learning,memory and antioxidative abilities of rats with Alzhheimer’s disease(AD)induced by D-galactose combined with amyloid β-protein(Aβ25-35 ),and provide experimental basis for the prevention and treatemtn of AD. Methods A total of 90 SD male rats were randomly divided into model control group,piracetam group,sham operated group,Bunao capsule(0.79,1.58,3.15 g·kg-1 )groups(n= 15 each).The rat models were established by intraperitoneal injection of D-galactose and injection of Aβ25-35 into the bilateral lateral cerebral ventricle.Then rats were given corresponding drugs by gavage in different groups for 8 weeks.The learning and memory abilities were meseured by Morris water maze test.The morphology of brain cells was observed by HE staining.The activities of glutathione peroxidase(GSH-Px)and superoxide dismutase( SOD),and the malondialdehyde( MDA)contents in the brain tissues were measured by spectrophotometry. Results The target quadrant residence time was(20.39±7.75)s and(20.82±5.09)s in Bunao capsule (1.58,3.15 g·kg-1 )groups,which were significantly increased as compared with that in model control group[(12. 35 ± 6.95)s](P<0.01).Brain nerve cell morphology in Bunao capsule(1.58,3.15 g·kg-1 )groups was obviously improved as compared with that in model control group,and was close to that in sham operated group.The activities of GSH-Px and SOD were significantly increased,and MDA contents decreased in Bunao capsule groups as compared with those in model control group (P<0.01). Conclusion Bunao capsule can dose-dependently improve the learning,memory and antioxidative abilities of AD rats.The mechanism may involve upregulation of antioxidative enzyme activities and removal of oxidative products.关键词
补脑胶囊/阿尔茨海默病/记忆能力Key words
Bunao capsule/Alzhheimer′s disease/Memory ability分类
医药卫生引用本文复制引用
贺勇,李玲,熊英,张金娟,陈燕..补脑胶囊对阿尔茨海默病模型大鼠学习记忆和抗氧化能力的影响[J].医药导报,2016,35(5):454-457,4.基金项目
贵州省中医药管理局中医药、民族医药科学技术研究项目 （）
