中国临床药理学杂志2016，Vol.32Issue(6)：531-533,3.DOI:10.13699/j.cnki.1001-6821.2016.06.017
粉防己碱对小鼠肺成纤维细胞的凋亡及 Caspase－3蛋白的影响
Effects of tetrandrine on apoptosis and Caspase -3 protein expression in mouse lung fibroblast
摘要
Abstract
Objective To explore the effect of tetrandrine ( Tet ) on the apoptosis expression of and Caspase-3 protein in mouse lung fibroblast cells( L929).Methods Lung fibroblast cells( L929 ) lines were incuba-ted in 50 mL · L-1 CO2 cells incubtion box at 37 ℃ for passage cul-ture.Fibroblast cells were divided into four group:blank group and diffe-rent does Tet(2.5,5.0,7.5 mg· L-1)groups which were cultured in Roswell Park Memorial Institute1640 medium without fetal bovine serum, penicilin and streptomycin.The cells were collected after 24 h,then mor-phological changes of the cells were observed under the transmission elec-tron microscope.The cell apoptosis was detected by the flow cytometry. And the expression levels of Caspase -3 protein were examined with immunohistochemistry method.Results The apoptotic cells increased while the number of cells decreased in different Tet concentrationgs group,when compared with the blank group.There were significant diffe-rent in the apoptotic cells compared with blank groups ( P<0.05 ).The apoptosis rate of blank group were (5.32 ±0.50)%,different does of Tet were ( 7.00 ±0.48 )%, ( 9.66 ±0.76 )%, ( 20.36 ±1.11 )%.There was significant different in the Caspase-3 expression among the differentgroups ( P <0.05 ) , and as Tet dosage increased, the expression of Caspase -3 protein increased ( P <0.05 ).Conclusion Tet can promote the apoptosis of mouse lung fibroblast cells and increase the expression of Caspase-3 protein so that the lung fibroblast was inhibited.关键词
粉防己碱/肺成纤维细胞/凋亡/Caspase-3蛋白Key words
tetrandrine/lung fibroblast cells/apoptotic/Caspase-3分类
医药卫生引用本文复制引用
梁晓飞,王铭,肖永红..粉防己碱对小鼠肺成纤维细胞的凋亡及 Caspase－3蛋白的影响[J].中国临床药理学杂志,2016,32(6):531-533,3.基金项目
河北省教育厅科学技术研究基金资助项目 （）
