中国临床药理学杂志2016，Vol.32Issue(6)：540-542,3.DOI:10.13699/j.cnki.1001-6821.2016.06.020
吉西他滨诱导胰腺癌 PANC－1细胞耐药与C－IAP2表达关系的研究
Gemcitabine-induced chemotherapy resistance of PANC-1 pancreatic cancer cell line and its association with C-IAP2 expression
摘要
Abstract
Objective To study the relationship between C-IAP2 ex-pression and gemcitabine(Gem) induced chemotherapy resistance of PANC-1 pancreatic cancer cell line.Methods PANC -1 was obtained by gradient increase of concentration.Semi inhibitory concentration ( IC50 ) of the cells was detected by MTT assay.PANC-1 and PANC-1/Gem cell doubling time was analyzed by cell counting assay.Cell cycle was analyzed by flow cytometry.C -IAP2 immunofluorescence expression was analyzed by immunofluorescence.C -IAP2 protein expression was detected by Western blot.Results IC50 value of PANC-1 /Gem was significantly higher than that of PANC-1 ( P<0.05 ).PANC-1/Gem doubling time was significantly longer than that of PANC-1 ( 32 h vs 23 h).There was No significant difference between PANC -1/Gem and PANC-1 in S phase.Compared with PANC-1, PANC -1/Gem ex-pressed the C-IAP2 with a significantly enhanced immunofluorescence, the expression levels of C -IAP2 significantly increased ( P <0.05 ).Conclusion Stable drug resistant of PANC-1/Gem cell strain can be obtained by intermittent incremental concentrations of gemcitabine.The up-regulated expression of C-IAP2 may play a role to some extent in the process of drug resistance.关键词
胰腺癌/细胞凋亡抑制蛋白2/多药耐药/吉西他滨Key words
pancreatic cancer/inhibitor of apoptosis protein-2/multiple drug resistance/gemcitabine分类
医药卫生引用本文复制引用
张帆,黄鹤光..吉西他滨诱导胰腺癌 PANC－1细胞耐药与C－IAP2表达关系的研究[J].中国临床药理学杂志,2016,32(6):540-542,3.基金项目
福建省自然科学基金资助项目 （）
