吕少君 1翁浩 2刘海健 3齐乐 3徐志勇 3刘荣 3黄丁丁3
作者信息
- 1. 南方医科大学 附属第三临床医学院，广州 510515
- 2. 南方医科大学附属奉贤医院麻醉科，上海 201400
- 3. 南方医科大学附属奉贤医院麻醉科，上海 201400
- 折叠
摘要
Abstract
Objective To evaluate of dexmedetomidine in rabbit spinal cord ischemia reperfusion injury proinflammatory cytokine tumor necrosis factor-α( TNF-α) , interleukin-1β( IL-1β) effect.Methods Twenty -four New Zealand white rabbits were randomly divided into three groups:sham operated group(n=8), model group(n=8) and ex-perimental group(n=8).The rabbit spinal cord ischemia again perfusion model was established, before ischemia 30 min vein infusion of dexme-detomidine 2.5μg· kg-1 until reperfusion before in experimental group.The model group and sham operation group, and the same time was pumped into the same amount of normal saline.After reperfusion, 6, 12, 24 and 48 h were used to evaluate the motor function of the hind limbs of the rabbits by Tarlov method.The expression levels of TNF-α, IL-1βat the time of 6 ,12 ,24 ,48 h were detected after surgery.The TNF-α, IL -1β, superoxide dismutase ( SOD ) and malondialdehyde ( MDA ) were detected and the pathological changes were observed by HE staining and 48 h staining.Results Compared with the sham operation group, the Tarlov score of the model group and the experimental group wasdecreased, serum and spinal cord TNF-α, IL-1βincreased, MDA,SOD decreased(all P<0.05), HE staining pathological damage aggravated.Compared with the model group, the Tarlov score of the experimental group was high-er, serum and spinal cord TNF--α, IL-1βdecreased, spinal cord MDA and SOD decreased (all P<0.05).The pathological damage of HE staining was decreased.Conclusion Dexmedetomidine can alleviate the rabbit spinal cord ischemia reperfusion injury, its mechanism may be associated with proinflammatory factor.关键词
右美托咪定/脊髓/缺血再灌注损伤/白细胞介素-1β/肿瘤坏死因子-αKey words
dexmedetomidine/spinal cord/ischemia-reperfusion/interleukin-1β/tumor necrosis factor-α分类
医药卫生
