中国临床药理学杂志2016，Vol.32Issue(7)：622-624,3.DOI:10.13699/j.cnki.1001-6821.2016.07.015
低分子量姬松茸多糖抑制氧应激大鼠海马神经元的凋亡作用
Inhibition function of low molecular weight polysaccharide from Agaricus blazei on the apoptosis of hippocampal neurons in rats with oxygen stress
摘要
Abstract
Objective To investigate the effect of low molecular weight polysaccharide from Agaricus blazei ( LMPAB ) on the expression of Bcl-2 and Bax in rat primary hippocampal neurons ( PHN) by oxidative stress.Methods PHN were clipped from 24 h -old rat and cultured primarily.Different groups were set, including the control group, model group and test groups (5,10 and 20 mg· L-1 ).At 24 h after LMPAB pretreatment, oxidative damage of cells were induced with 500μmol· L-1 H2 O2 for 2 h in model and test group.The PHN apoptosis and cell activity were measured with flow cytometry and MTT method.The expression of Bcl-2 and Bax protein were detected by immunocyto-chemical technique.Results Hippocampus neuron apoptosis rate in high dose test group (10.08 ±0.83) was significantly lower than that of model group ( 29.01 ±0.67 , P <0.01 ).Cell viability in high dose group ( 0.38 ±0.05 ) was significantly higher than that of model group (0.20 ±0.07, P <0.01 ) .Compared with model group, Bcl -2 expression significantly increased ( 42.73 ±3.65 vs 29.43 ±2.26 ).However, Bax expression significantly reduced in high dose group (22.47 ±2.82 vs 47.58 ±4.79)(P <0.01), Bcl -2/Bax increased.Conclusion LMPAB attenuated the hippcampal neurons oxidative damage induced by H2 O2.The mechanism may be related to its inhibition on the apoptosis of hippocampal neurons.关键词
海马神经元/氧化应激/低分子姬松茸多糖/细胞凋亡Key words
hippocampal neurons/oxidative stress/low molecular weight polysaccharide from Agaricus blazei/cell apoptosis分类
医药卫生引用本文复制引用
樊丽,李世玲,伊超,周丽,邹宇,李刚,岳丽玲..低分子量姬松茸多糖抑制氧应激大鼠海马神经元的凋亡作用[J].中国临床药理学杂志,2016,32(7):622-624,3.基金项目
黑龙江省教育厅科学技术研究基金资助项目 （）
