中国临床药理学杂志2016，Vol.32Issue(8)：696-699,4.DOI:10.13699/j.cnki.1001-6821.2016.08.008
阿奇霉素对白细胞介素－22诱导的 HaCaT角质形成细胞炎症干预作用
Inhibition function of azithromycin on inflammation in HaCaT keratinocyte cell induced by interleukin-22
摘要
Abstract
Objectives To explore the inhibition function of azithromycin on inflammation function in HaCaT keratinocyte cell induced by interleukin-22 (IL-22).Methods The cases were randomly divided into normal group, model group (100 μg・ mL-1 IL-22) , azithromycin low, medi-um and high dose groups (100, 300, 1000 μg・ mL-1 ).The content of nitric oxide ( NO) in medium was determined by Gries method.The con-tent of tumor nuclear factor ( TNF-α) , IL-6, IL-6 was examed by enzyme linked immunosorbent assay ( ELISA) .The phosphorylation of nuclear factor kappa B ( NF-κB) p65 and the expression of inducible nitric oxide synthase ( iNOS ) was assayed by Western blot.The expre-ssion of NF-κB p65,iNOS,TNF-α,IL-6,IL-8 mRNA was assayed by reverse transcription polymerase chain reaction ( RT -PCR ) . Results Compared with model group, the concentration of NO, the expression of TNF-α, IL-6 , IL-8 protein and mRNA was lower than in three dose azithromycin groups ( P <0.05 ) . The expression of TNF-α, IL-6 protein in model group and azithromycin medium group were [(39.12 ±3.45) vs (16.37 ±1.28)], [( 30.42 ±2.97) vs (13.29 ±1.52)].The expression of TNF-α, IL-6 mRNA in model group and azithromycin medium group was [(1.28 ±0.11) vs (0.72 ±0.07)],[( 0.89 ±0.08) vs (0.53 ±0.04)].The expression of iNOS protein and mRNA, the phosphorylation of NF-κB p65 and the expression of NF-κB p65 mRNA was lower than in azithromycin medium and high dose groups ( P <0.05 ) . Conclusion The azithromycin could inhibit inflammation in HaCaT keratinocyte cell induced by IL -22, may be correlated with via blocking NF -κB signal pathway and down-regulation expression of iNOS.关键词
阿奇霉素/白细胞介素-22/HcaTa角质形成细胞/细胞炎症Key words
azithromycin/interleukin-22/HaCaT keratinocyte cell/cell inflammation分类
医药卫生引用本文复制引用
陈庆宁,王小川,杜鹏,金梅,樊楚明..阿奇霉素对白细胞介素－22诱导的 HaCaT角质形成细胞炎症干预作用[J].中国临床药理学杂志,2016,32(8):696-699,4.基金项目
云南省科技厅昆明医科大学联合基金资助项目 （）
