中国临床药理学杂志2016，Vol.32Issue(8)：717-719,3.DOI:10.13699/j.cnki.1001-6821.2016.08.014
丁苯酞对急性重度一氧化碳中毒大鼠神经元凋亡相关因子表达的影响
Effects of dl-3-n-butylphthalide on expression of apoptosis-related factors in rats with acute severe carbon monoxide poisoning
摘要
Abstract
Objective To investigate the effects of dl -3 -n -butylphthalide(NBP) on the expression of B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein ( Bax) in the rat brain tissue after acute severe carbon monoxide ( CO) poisoning.Methods Rats were random-ly assigned into three groups:normal group, CO-exposed group ( model group) and NBP ( test group ) . The acute severe carbon monoxide poisoning model was made by the ischiropoulos method.The rats in the test group were lavaged with 80 mg・ kg-1 NBP after making a model. The levels of Bcl -2 and Bax expression were measured by immuno-histochemistry, RT-PCR and Western blot.Results Bcl-2 and Bax have low level of expression in normal group.After acute severe carbon monoxide poisoning, the expression of Bcl-2 and Bax positive cells were (22.62 ±4.39),(38.65 ±7.54), indicating that mRNA and protein in-creased significantly in rats.Compared with model group, in rats of test group, the expression of Bcl-2 positive cells was (35.13 ±6.69), the mRNA and protein were up regulated( P<0.05) , while the expression of Bax positive cells was ( 27.36 ±5.57 ) , mRNA and protein were down regulated ( P<0.05).Conclusion NBP can relieve the brain damage partly by regulatng Bcl-2 and Bax synthesis in brain.关键词
丁苯酞/一氧化碳中毒/B细胞淋巴瘤基因-2/Bcl-2相关X蛋白Key words
dl-3-n-butylphthalide/carbon monoxide poisoning/B-cell lymphoma-2/Bcl-2 associated X protein分类
医药卫生引用本文复制引用
洪诸权,王杰华,李国前,杨小霞,潘莹..丁苯酞对急性重度一氧化碳中毒大鼠神经元凋亡相关因子表达的影响[J].中国临床药理学杂志,2016,32(8):717-719,3.基金项目
2014年泉州市卫生科研基金资助项目 （）
