孙福东 1冯乃杰 1郑殿峰 1崔洪秋 1刘春娟 1何天明 1赵晶晶1
作者信息
- 1. 黑龙江八一农垦大学农学院化控研究室/黑龙江省高校寒地作物种质改良与栽培重点实验室，黑龙江大庆 163319
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摘要
Abstract
[Objective] Pods are important source organs for temporary reserve photosynthate in soybean grain growth progress. It is a key factor of pods abscission to restrict soybean production. In order to investigate the internal mechanism of chemical control technology to improve soybean yield, this study used two plant growth regulators to discover the different effects on physiological metabolism in pods and the expression of abscission cellulose enzyme gene (GmAC) in the abscission zone. This study will provide a theoretical basis for high-yield, high-quality and high-efficiency in soybean production.[Method]Field experiments were conducted with soybean Kangxian 6. We sprayed growth promoter regulator 2-N,N-diethylamino ethyl caproate (DTA-6) 60 mg·L-1 and the retardant regulator uniconazole (S3307) 50 mg·L-1 or water during R1 growth stage in 2012 and 2013. The pods of each treatment were used to examine pod physiological indicators including soluble sugar, sucrose, starch, MDA, POD and AC on 35, 42, 49 and 56 days after spraying in each treatment. Abscission zone tissues between stem and soybean pods were cut on the 5th day after treatment, to determinate the expression of cellulose enzyme gene (GmAC) using applied RT-PCR method. [Result](1) Compared with the control, DTA-6 and S3307 treatments had significantly lower soluble sugar, except on 35 d after spraying where the DTA-6 treatment sugar content was higher. The content of sucrose in pods decreased after spraying DTA-6 and S3307 from the 35 d to the 49 d, and increased on the 56 d after spraying. Starch increased in most of treatment-spray time conditions except in the S3307 treatment on the 42 d after spraying. (2) The content of MDA decreased after 35, 49 and 56 d after spraying. The activity of POD increased after 35 d, 42 d and 56 d after spraying. The activity of AC decreased on the 35 d, 49 d and 56 d after spraying S3307 and decreased from 42 d to 49 d after spraying DTA-6. (3) The relative expression of GmAC in abscission zone after spraying DTA-6 was down-regulated, but the relative expression after spraying of S3307 was up-regulated. (4) DTA-6 and S3307 treatments effectively improved yield properties such as the number of pods per plant, grains per pod and one-hundred grain weight, compared with control. The yield improved by 6.2%and 1.4%in 2012, and 4.8%and 5.3%in 2013.[Conclusion]Our observations suggest that DTA-6 and S3307 increased transportation and accumulation of assimilate in pods, enhanced the antioxidant enzyme, reduced membrane lipid peroxidation products, decreased the key enzyme activity of abscission pods, benefited the pods, and ultimately improved the production of soybean. The relative expression of GmAC was down-regulated in the treatment with DTA-6, and DTA-6 treatment was better.关键词
大豆/荚/植物生长调节剂/生理代谢/基因表达Key words
soybean/pod/plant growth regulator/physiological metabolism/gene expression
