摘要
Abstract
[Objective]Experiments were carried out to study the physiological status of harvested Satsuma mandarin and to discover representative biomarker candidates that represent the status of citrus fruit. [Method]Based on the data of microarray and primary metabolites of Satsuma mandarin during 0-50 DAH (days after harvest), approaches of hierarchical clustering, principal component analysis, partial least squares discriminant analysis and support vector machine model were integratively used to assess different physiological status and to discover representative biomarker candidates representing citrus fruit storage process. Additionally, the high reliability of transcriptome data was verified by qRT-PCR. [Result]A total of 5 232 differentially expressed genes were identified. The post-harvest storage process of Satsuma mandarin could be classified into early stage, middle stage and terminal stage according to the differentially expressed genes. Functional categorization by Mapman showed that the biological process of Satsuma mandarin during storage was closely related to amino compound metabolism and cell wall metabolism. The statistical analysis of gene ontology revealed that nucleosome assembly genes and proteolysis genes were overrepresented. Combined with the verification of qRT-PCR, the expression trend of glutathione S-transferase had obviously stage characteristics, and therefore it can be used as a biomarker evaluating Satsuma mandarin post-harvest storage status. The same classification of physiological stages of Satsuma mandarin during storage was done according to the primary metabolites data. Further, four candidate metabolite biomarkers, including Glycine, 3-Amino-6-methoxypicolinic acid, 2-Butenedioic acid and Cyclohexanone,2-[(dimethylamino)methyl] were screened.[Conclusion]Five biomarkers, including glutathione S-transferase, glycine, 3-amino-6-methoxypicolinic acid, cyclohexanone,2-[(dimethylamino)methyl], 2-butenedioic acid were screened, which could be used to predict the storage life of harvested Satsuma mandarin.关键词
温州蜜柑/果实贮藏/生物标记/基因芯片/初生代谢物Key words
Satsuma mandarin/fruit storage/biomarkers/microarray/primary metabolites
