中国社区医师2016，Vol.32Issue(13)：7-8,10,3.DOI:10.3969/j.issn.1007-614x.2016.13.1
Percoll及Ficoll分离纯化肝癌移植瘤肿瘤浸润淋巴细胞比较
Comparison of Percoll and Ficoll isolated and purified tumor infiltrating lymphocytes in hepatocellular carcinoma
摘要
Abstract
Objective:To compare the effects of the two kinds of Percoll and Ficoll lymphocytes on the purity and cell proliferation of tumor infiltrating lymphocytes.Method:H22 mice were isolated subcutaneous transplantation tumor lymphocyte by Percoll and Ficoll two kinds of lymphocytes.The cell count and survival rate were calculated under microscope after the cells were first stained with a cell line,and the purity and proliferation ability of lymphocytes were analyzed by flow cytometry.Results:The total number of Percoll and Ficoll isolated from TIL respectively(3.28±0.78)×107 and(2.02±0.76)×107;the TIL proliferation rate of the 3d,5d,7d through Percoll separating income were(22.8±2.31%),(39.1±1.94%),(69.85±1.68)% respectively;through the Ficoll separating income were (13.25±1.68)%,(23.7±5.31)% and(41.5±1.56)% respectively.The CD3+T,CD4+T,CD8+T cells in TIL through Percoll separating income were(73.2±6.8%),(49.1±10.2%),(22.0±1.4)%;the CD3 +T,CD4 +T,CD8 +T cells in TIL through Ficoll separating income were(47.9 ± 5)% and(29.1 ± 8.4)% and(19.7 ± 5)% .Conclusion:Compared with Ficoll,the separation of Percoll H22 mice transplanted TIL cell yield and purity is higher,stronger proliferation ability,which will be more conducive to the follow-up study.关键词
肿瘤浸润淋巴细胞/淋巴细胞分离液/密度梯度分离/肝癌移植瘤Key words
Tumor infiltrating lymphocytes/Lymphocyte separation solution/Density gradient separation/Liver tumor引用本文复制引用
孙盼,樊晓晖,张彬,张磊,蔡双启,梁莹,赖振屏,高灵茜,宋德志..Percoll及Ficoll分离纯化肝癌移植瘤肿瘤浸润淋巴细胞比较[J].中国社区医师,2016,32(13):7-8,10,3.基金项目
国家自然科学基金项目(81460437)；广西自然科学基金青年基金项目(2013GXNSFBA019160)；广西科技技术开发项目(桂科合1347004-27)；教育部博士导师联合基金项目(20124503110007)；广西特聘专家岗位科研项目(2013-2016)；广西教育厅科研立项项目(201204LX032)；广西卫生厅课题项目，桂卫自筹Z2013045；广西壮族自治区卫生和计划生育委员会科研课题Z2015500 （81460437）
