中国实验动物学报2016，Vol.24Issue(2)：158-163,6.DOI:10.3969/j.issn.1005-4847.2016.02.009
小鼠脓毒症模型的建立和评价
Establishment and evaluation of a mouse model of sepsis
摘要
Abstract
Objective The purpose of this study was to establish and evaluate a mouse model of sepsis for studying the mechanism of sepsis and development of anti-inflammatory drugs.Methods The sepsis in mice was induced by cecal ligation and puncture ( CLP) .The survival rates, microbial load, liver and kidney damages, cytokines and pathological changes were detected to evaluate the mouse models.Results The death of mice was closely related with the ligated sites. The mice with 50%cecal ligation displayed about 40% of 12-day survival rate, however, all the mice with 75% cecum ligation died within 4 days (P<0.01).Compared with the sham surgery group, the mice with 50% cecal ligation had a high microbial load in the blood and abdominal cavity.Leukopenia was also emerged (P<0.001).CLP mice demonstra-ted elevated levels of serum ALT, AST and BUN (P<0.01).The levels of IL1α, IL6, IL10, MIP1α, MIP1β, and TNFαwere increased a lot.The liver and lung showed obvious pathological injury at 48 h post CLP.Conclusions The established mouse model of CLP shows typical characteristics of sepsis and is an ideal tool for further study of anti-inflam-matory drugs.关键词
脓毒症/盲肠结扎穿孔/小鼠模型/炎症反应/感染Key words
Sepsis/CLP/Mouse model/Inflammatory response/Infection分类
生物科学引用本文复制引用
荆喜中,王晖,贾欢欢,罗挺,凌雪荧,李韵峰,刘书华,马俊峰,黄韧,张钰..小鼠脓毒症模型的建立和评价[J].中国实验动物学报,2016,24(2):158-163,6.基金项目
广东省科技计划项目（2015A030302034）。 （）
