中国比较医学杂志2016，Vol.26Issue(4)：41-45,5.DOI:10.3969/j.issn.1671-7856.2016.04.007
CEMx174细胞中 SAMHD1蛋白表达与SIVmac239病毒水平的相关性研究
Relationship between the SAMHD1 protein expression and the SIVmac239 viral level in CEMx174 cells
摘要
Abstract
Objective To explore the relationship between protein levels of SAMHD1 expression with viral load of SIV in the SIVmac239-infected CEMx174 cells.Methods CEMx174 cells were infected with SIVmac239, and the cells and supernatant were collected daily for analysis.The viral loads and the SAMHD1 mRNA level were detected by real-time RT-PCR and the level of P27 and SAMHD1 proteins were detected by Western blotting.Results The mRNA level of intracellular SAMHD1 gene was increased and SAMHD1 protein decreased gradually when CEMx174 cells were infected with SIVmac239.Conclusions There is a positive correlation between viral load in the supernatant and the mRNA level of SAMHD1, and a negative correlation between the increase of P27 protein and the decrease of SAMHD1 protein in the SIVmac239-infected CEMx174 cells.关键词
SAMHD1/HIV-1/SIV/实时荧光定量RT-PCRKey words
SAMHD1/HIV-1/SIV/Real-time RT-PCR分类
医药卫生引用本文复制引用
彭灵娟,丛喆,薛婧,宋铭晶,魏强..CEMx174细胞中 SAMHD1蛋白表达与SIVmac239病毒水平的相关性研究[J].中国比较医学杂志,2016,26(4):41-45,5.基金项目
国家十二五科技重大专项课题（2013ZX10004608－003和2012ZX10004501－001）。 （）
