中国比较医学杂志2016，Vol.26Issue(4)：46-52,7.DOI:10.3969/j.issn.1671-7856.2016.04.008
基于实时细胞分析技术检测前列腺癌细胞药物敏感性
Assessment of drug sensitivity of the prostate cancer cells based on real-time cell analysis system
摘要
Abstract
Objective To develop a more convenient and stable method for assessment of drug sensitivity of prostate cancer based on real-time cell analysis system as a reference for clinical treatment.Methods Human prostate cancer VCaP, DU145, PC-3, PC-3M-2B4 and PC-3M-IE8 cells were chosen to detect the sensitivity to three drugs, docetaxel, cabazitaxel and abiraterone acetate.Serial dilutions of the three drugs were used to treat the cell culture for 24 hours.The drug-induced effects on the cell lines after an incubation of 24 hours were recorded by the real-time cell analysis system to determine the half maximal inhibitory concentration (IC50).Results Docetaxel showd IC50 of 8.81 nmol/L, 11.61 nmol/L, 1.78 nmol/L, 1.44 nmol/L, 8.69 nmol/L for VCaP, DU145, PC-3, PC-3M-2B4, PC-3M-IE8 cells, respectively.Cabazitaxel showed IC50 of 3.73 nmol/L, 3.96 nmol/L, 10.41 nmol/L, 5.43 nmol/L, and 7.37 nmol/L, respectively, for the five cell lines.Abiraterone acetate showed IC50 of 8.34 μmol/L, 8.60 μmol/L, 24.20 μmol/L, 8.59 μmol/L, and 13.21 μmol/L for the five cell lines.Conclusions PC-3M-2B4 and DU145, VCaP and PC-3 cells can be used as control for docetaxel, cabazitaxel and abiraterone acetate to establish cell models for the drug screening in vitro and to provide reference for clinical applications.关键词
前列腺癌/化疗药物/实时细胞分析/药物敏感性Key words
Prostate cancer/Chemotherapy drugs/Real-time cell analysis system/Drug sensitivity分类
医药卫生引用本文复制引用
靳亚西,孙彩显,高虹,张连峰,张丽..基于实时细胞分析技术检测前列腺癌细胞药物敏感性[J].中国比较医学杂志,2016,26(4):46-52,7.基金项目
卫生行业科研专项（201402001）。 （）
