中国比较医学杂志2016，Vol.26Issue(4)：62-67,6.DOI:10.3969/j.issn.1671-7856.2016.04.011
改良的乳小鼠心肌细胞原代培养方法
An improved method for primary culture of neonatal mouse cardiomyocytes
摘要
Abstract
Objective To establish a stable and fast method for primary culture of mouse cardiomyocytes. Methods Dishes were coated with polylysine firstly.A two-step approach was used to isolate and digest mouse cardiomyocytes cells (0.25%trypsin in 4°C overnight and 0.5 mg/mL to 1.0 mg/mL collagenase +5 mg/mL albumin collagen digestion liquid in 37°C for short-time digestion), then the cardiomyocytes were purified through differential adhesion for 70 min and 5-bromodeoxyuridine ( BrdU) .The cell morphology was observed under an inverted microscope. The survival rate of cardiacmyocytes was detected by trypan-blue staining and their purity was identified by α-actinin immunofluorescence staining.Results The cardiomyocytes were in good shape and pulsed spontaneously.The survival rate of the cardiomyocytes reached 98%and the purity was 95%.Conclusions This method described in this study is an ideal method for primary culture of mouse cardiomyocytes with a high survival rate and high purity.关键词
乳小鼠/心肌细胞/原代培养Key words
Neonatal mouse/Cardiomyocytes/Primary cell culture分类
医药卫生引用本文复制引用
吴丹,冯健,莫显刚,刘应才..改良的乳小鼠心肌细胞原代培养方法[J].中国比较医学杂志,2016,26(4):62-67,6.基金项目
国家自然科学基金（编号31300946，31260250），沪州市人民政府－沪州医学院科技战略合作科技项目（2013LZLY-J22）。 （）
