中国比较医学杂志2016，Vol.26Issue(4)：75-78,85,5.DOI:10.3969/j.issn.1671-7856.2016.04.014
改良组织块法分离培养兔成纤维样滑膜细胞
Primary culture of rabbit fibroblast-like synoviocytes using an improved explant culture method
陈芳 1刘琴 1王丽平 1陈利峰 2张宜1
作者信息
- 1. 解放军广州军区武汉总医院医学实验科，武汉 430070
- 2. 解放军广州军区武汉总医院中西医结合科，武汉 430070
- 折叠
摘要
Abstract
Objective To establish an easier and better method for primary culture of rabbit fibroblast-like synoviocytes(FLS) by modified tissue cell culture.Method Healthy rabbit joint synovial layers were cut into small pieces and siphoned off water attached on the tissue with sterile filter paper and then cultured.The growth status and morphology were observed.The growth curve of the 3rd passage cells was measured by CCK-8 assay, and the expression of vimentin was detected by immunocytochemistry.Results The FLS cultured in vitro exhibited a spindle-shaped appearance and could rapidly expand.The cell growth curve was typical of S type, and the cells highly expressed vimentin.Conclusions Primary culture of rabbit FLS by the improved explant culture method is successfully established.The method is simple and highly efficient.It provideds a new method for isolation of FLS.关键词
改良组织块法/兔成纤维滑膜细胞/分离/培养Key words
Improved explant culture method/Rabbit fibroblast-like synoviocytes/Isolation/Culture分类
医药卫生引用本文复制引用
陈芳,刘琴,王丽平,陈利峰,张宜..改良组织块法分离培养兔成纤维样滑膜细胞[J].中国比较医学杂志,2016,26(4):75-78,85,5.
