中国医学前沿杂志（电子版）2016，Vol.8Issue(3)：108-112,5.
共价结合生长因子胶原补片对人骨髓间充质干细胞增殖和分化的影响
Covalent binding of growth factor collagen patch on human bone marrow mesenchymal stem cell proliferation and differentiation
卢耀军 1赵瑞平 1孙凯 1乌日娜 1韩瑞娟1
作者信息
- 1. 包头市中心医院 心内科，内蒙古 包头014040
- 折叠
摘要
Abstract
ObjectiveTo prepare the basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) collagen covalently bonded patch, and discusse the effects of human bone marrow mesenchymal stem cell proliferation and differentiation.MethodCollagen patch was prepared and were randomly divided into control group and experimental group. Control group collagen patch treated by carbodiimide under sterile conditions. Experimental group collagen patch at room temperature under the condition of used N-hydroxy succinimide and+phosphate buffered saline+EDC.HCL and with bFGF and VEGF of covalent binding. Saved after 1, 3, 7, 14, 21, 28 days, the concent of bFGF and VEGF in the supernatant lfuid were detected by ELISA. The human bone marrow mesenchymal stem homogeneous cell were seeded on two groups of collagen patch, cell counts and MTT assay and bromodeoxyuridine monoclonal antibody proliferation assay were performed, and the SMA immunolfuorescence staining method was used to detect cell differentiation, the expression of collagenⅠ and collagen Ⅲ were detected by RT-PCR.Result Experimental group collagen patch with VEGF and bFGF binding were signiifcantly higher than control group (P<0.05). Draw the release curve and analysis found that the experimental group growth factor in a very stable state, only a handful of dissociated release. 28 days after preparation, most growth factor remained in the collagen patch. Two groups of collagen patch with the passage of time, bone marrow mesenchymal stem cells showed a growth trend. 3 and 7 days after inoculation comparison showed that experimental group collagen patch on carbodiimide were signiifcantly more than control group (P<0.05). Compared with control group, experimental group of collagen supplement cell sheet on the expression levels of collagenⅠ and collagenⅢwere signiifcantly reduced by RT-PCR detection and SMA staining found (P<0.05).ConclusionbFGF and VEGF covalent binding of collagen patch has a greater impact on human bone marrow mesenchymal stem cells, and can promote the proliferation and inhibition of differentiation effectively.关键词
胶原蛋白海绵/生长因子/碱性成纤维细胞生长因子/血管内皮细胞生长因子/人骨髓间充质干细胞/增殖/分化Key words
Collagen sponge/Growth factor/Basic ifbroblast growth factor/Vascular endothelial growth factor/Mesenchymal stem cells/Proliferation/Differentiation引用本文复制引用
卢耀军,赵瑞平,孙凯,乌日娜,韩瑞娟..共价结合生长因子胶原补片对人骨髓间充质干细胞增殖和分化的影响[J].中国医学前沿杂志（电子版）,2016,8(3):108-112,5.
