中国中医药信息杂志2016，Vol.23Issue(5)：69-72,4.DOI:10.3969/j.issn.1005-5304.2016.05.018
党参水提物对D-半乳糖致衰老小鼠肾组织 microRNA表达谱的影响
Effects of Aqueous Extracts of Codonopsis Radix on MicroRNA Expression Profiling of D-galactose Induced Kidney of Aging Mice
摘要
Abstract
Objective To explore the effects of aqueous extracts of Codonopsis Radix onD-galactose induced aging model mice; To discuss the anti-aging molecular mechanism of Codonopsis Radix.Methods Subcutaneous injection ofD-galactose solution was used to establish aging models. 100 Kunming mice were divided into normal control group, model group and low-, medium-, high-dose of Codonopsis Radix interventional groups randomly, 20 mice in each group. Low-, medium-, high-dose of Codonopsis Radix interventional groups were given relevant dosage for gavage, while normal control group and model group were given the same volume of NS by gavage for 42 d. After treatment for 42 days, the BUN and CREA in mouse serum were examined; the AffymetrixmiRNA 4.0 microarray was employed to identify the differentially expressed microRNA (miRNA) related with these processes; the bioinformatic tools were also used to further analyze the cluster of miRNA microarrys and the pathways which the target genes of miRNA were involved in.Results Compared with normal control group, the levels of BUN and CREA in mouse serum increased in model group (P<0.05); compared with model group, the levels of BUN and CREA in Low-, medium-, high-dose of Codonopsis Radix interventional groups decreased, in which high-dose of Codonopsis Radix could most significantly inhibit the level of BUN (P<0.05); the cluster analysis showed the miRNA expression profilings of high dose of Codonopsis Radix interventional group and normal control group were brought together, while the profiling of model group was clearly divided with the other groups. In model group vers normal control group, 36 differentiated expressed miRNA showed, which predicated in 10 main biological functions. In high-dose of Codonopsis Radix interventional group vers model group, 34 differentiated expressed miRNAs in high-dose of Codonopsis Radix interventional group showed, and the analytical results of biological functions of target genes were the same as those of model group vers normal control group.Conclusion In the anti-aging process, Codonopsis Radix can not only influence the miRNA expression profiling, but also influence its functional environment.关键词
党参/抗衰老药/microRNA表达谱/芯片/小鼠Key words
Codonopsis Radix/anti-aging medicine/microRNA expression profiling/microarray/mice分类
医药卫生引用本文复制引用
王晶,黄勇,李海龙,林志艳,田一虹,陈彻..党参水提物对D-半乳糖致衰老小鼠肾组织 microRNA表达谱的影响[J].中国中医药信息杂志,2016,23(5):69-72,4.基金项目
甘肃省中医方药挖掘与创新转化重点实验室开放基金项目（ ZYFYZH-KJ-2015-008）；甘肃省高等学校科研项目 （）
