中国中医药信息杂志2016，Vol.23Issue(5)：104-106,3.DOI:10.3969/j.issn.1005-5304.2016.05.026
HPLC测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量
Content Determination of Protodioscin and Diosgenin in Dioscoreae Hypoglaucae Rhizoma from Different Habitats by HPLC
摘要
Abstract
Objective To establish an HPLC method for the content determination of protodioscin and diosgenin in Dioscoreae Hypoglaucae Rhizoma from different habitats.Methods The chromatographic separation was performed on an Agilent C18 column (4.6 mm×150 mm, 5μm) with mobile phase of acetonitrile-water solution with gradient elution at the flow rate of 0.8 mL/min; the detection wavelength was 208 nm; the column temperature was 30℃; the injection volume was 20μL.Results Protodioscin showed a good linear relationship among the range of 1.73–8.64 µg (r=0.999 6), with the average recovery of 101.98% (RSD=1.53%); Diosgenin showed a good linear relationship among the range of 1.03–8.20μg (r=0.999 1), with the average recovery of 101.60% (RSD=2.41%). The contents of protodioscin and diosgenin in Dioscoreae Hypoglaucae Rhizoma from 10 different habitats were in the range of 0.89%–2.24% and 0.75%–3.22%, respectively.Conclusion The method is simple, accurate and with repeatability, which can be used as quality control method of Dioscoreae Hypoglaucae Rhizoma.关键词
粉萆薢/中药产地/原薯蓣皂苷/薯蓣皂苷/高效液相色谱法Key words
Dioscoreae Hypoglaucae Rhizoma/place of production (TCD)/protodioscin/diosgenin/HPLC分类
医药卫生引用本文复制引用
邵岩岩,肖潇,吴玲芳,朱丹,杨光辉,张晓雪,张兰珍..HPLC测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量[J].中国中医药信息杂志,2016,23(5):104-106,3.基金项目
北京中医药大学科研创新团队项目 （）
