肿瘤药学2016，Vol.6Issue(2)：114-119,6.DOI:10.3969/j.issn.2095-1264.2016.02.06
MicroRNA-503抑制乳腺癌细胞增殖的作用及其机制研究
Effects of MicroRNA-503 on the Proliferation of Breast Cancer Cells and its Mechanism
摘要
Abstract
Objective To investigate the effects of miR-503 on the proliferation of breast cancer cells and its mechanism. Methods In the present study, we collected 35 paired breast cancer tissues and matched adjacent tissues, and their miR-503 expressions were detected by real-time fluorescence quantification PCR. T47D cells were transfected with miR-503 mimic and miR negative controls (miR-NC), respectively. Luciferase assay for candidate miR-503 target gene, insulin-like growth factor 1 receptor (IGF-1R), was performed, and the eukaryotic expression plasmids of this gene were transfected into T47D cells which were pretreated with miR-503 or miR-NC. Then activi-ties of all these treated cells were measured by MTT. Results Real-time PCR data showed that miR-503 was significantly downregulated in breast cancer tissues as compared to adjacent non-tumor tissues. Overexpression of miR-503 significantly suppressed the proliferation of breast cancer T47D cells. Bioinformatics analysis and luciferase reporter assay data indicated that insulin-like growth factor 1 receptor (IGF-1R) was a target gene of miR-503, and the protein expression of IGF-1R was negatively mediated by miR-503 in T47D cells. Moreo-ver, overexpression of IGF-1R remarkably reversed the suppressive effect of miR-503 on T47D cell proliferation, suggesting that the sup-pressive effect of miR-503 on T47D cells was directly targeting IGF-1R. Conclusion miR-503 inhibited the proliferation of breast cancer cells through its negative mediation on expression of IGF-1R. The miR-503/IGF-1R signaling may become a potential therapeutic target for breast cancer treatment.关键词
乳腺癌/MicroRNA-503/胰岛素样生长因子 1 型受体/增殖Key words
Breast cancer/MicroRNA/Insulin-like Growth Factor-1 Receptor/Proliferation分类
医药卫生引用本文复制引用
蒲涛,曹璐,梁琪,刘华生,杨明,叶斌..MicroRNA-503抑制乳腺癌细胞增殖的作用及其机制研究[J].肿瘤药学,2016,6(2):114-119,6.基金项目
湖南省科技计划社会发展支撑计划（2015SK20664）。 （）
