肿瘤药学2016，Vol.6Issue(2)：126-131,6.DOI:10.3969/j.issn.2095-1264.2016.02.08
沉默 TGFA 抑制肝癌细胞增殖的研究
Knockdown of TGFA Inhibits the Growth of Hepatocellular Carcinoma
罗嘉 1石磊 1贺卓 1陈杰 1吴飞跃1
作者信息
- 1. 湖南省肿瘤医院/中南大学湘雅医学院附属肿瘤医院 肝胆肠外科，湖南 长沙，410013
- 折叠
摘要
Abstract
Objective To investigate the effects of knockdown of TGFA on the proliferation of hepatocellular carcinoma cells. Methods We selected TGFA as the candidate target gene. TGFA-siRNA was transfected into L02 and HepG2 cells. The expression efficiency of TGFA mRNA was detected by Real-time PCR, and the expression level of TGFA protein was detected by Western blot. Then MTT and BrdU methods were used to detect the effects of down-regulation of TGFA-siRNA on proliferation of L02 and HepG2 cells. Western blot assay was applied to detect the TGFA/EGFR pathway activity. TGFA over expression was achieved to verify the correlation between TGFA and EGFR expression in hepatocellular carcinoma. Results TGFA-siRNA down-regulated the expression of TGFA, inhibited proliferation of human hepatoma cells but had no obvious effects on normal cell line L02. It also inhibited the activity of TGFA/EGFR pathways. The mRNA expression levels of TGFA and EGFR were specifically high in the hepatocellular carcinoma tissue and were positively correlated. Conclusion Knockdown of TGFA inhibited the proliferation of HepG2 hepatocellular carcinoma cells.关键词
TGFA/HepG2/增殖/EGFRKey words
TGFA/HepG2/Proliferation/EGFR分类
医药卫生引用本文复制引用
罗嘉,石磊,贺卓,陈杰,吴飞跃..沉默 TGFA 抑制肝癌细胞增殖的研究[J].肿瘤药学,2016,6(2):126-131,6.
