肿瘤药学2016，Vol.6Issue(2)：132-135,4.DOI:10.3969/j.issn.2095-1264.2016.02.09
Twist1基因在 A549与 A549/DDP细胞中的表达及意义
The Expression of Twist1 Gene in A549 and A549/DDP Cells and its Significance
摘要
Abstract
Objective To investigate the relationship between Twist1 and cisplatin resistance in lung adenocarcinoma by detecting the expression of Twist1 gene in A549 cell line and A549/DDP cell line. Methods The growth of A549 cells and A549/DDP cells was observed dynamically before and 24h after the intervention of cisplatin with different concentration gradients, and cells were counted at the same time. The A549 cells and A549/DDP cells were split by RIPA Lysis Buffer to release the cell total protein completely. The total protein concentration was detected by enhanced BCA Protein Assay Kit, and the concentration of Twist1 transcription factor was detected by ELISA assay. Results The `m' was defined as m =concentration of Twist1 transcription factor / concentration of total protein. The m of A549 cells and A549/DDP cells was 3.01/3.31, 2.17/2.82, 1.63/2.94, 1.73/1.99, 0.37/1.31, -/- respectively when intervened by 0, 10, 20, 30, 40, 50 mg·L-1 of cisplatin. The m of A549/DDP cells was significantly higher than that of A549 cells (P<0.05). Conclusion Twist 1 gene was highly expressed in human cisplatin-resistant lung adenocarcinoma cells. It may suggest a relationship between Twist1 and cisplatin resistance in lung adenocarcinoma.关键词
肺腺癌/顺铂/耐药性/Twist1 基因Key words
Lung adenocarcinoma/Cisplatin/Drug resistance/Twist1分类
医药卫生引用本文复制引用
程旭,刘颖,汤永昌,肖玲..Twist1基因在 A549与 A549/DDP细胞中的表达及意义[J].肿瘤药学,2016,6(2):132-135,4.基金项目
?基金项目中南大学大学生创新课题C类项目（CY14220）。 （）
