肿瘤药学2016，Vol.6Issue(2)：150-153,4.DOI:10.3969/j.issn.2095-1264.2016.02.13
连续100例肺癌术后 EGFR 基因突变检测结果分析
Analysis on the EGFR Mutation Test Results of 100 Sequential Patients with Primary Bronchogenic Carcinoma
摘要
Abstract
Objective To investigate the gene mutation characteristics of epidermal growth factor receptor (EGFR) and its relationship with clinical features (pathological pattern,gender, smoking history, clinical stage, differentiation) in primary bronchogenic carcinoma. Methods We tested mutation of EGFR exon 18, 19, 20 and 21 with PCR amplification and gene se-quencing in 100 sequential patients who had been diagnosed with primary bronchogenic carcinoma in Hunan Cancer Hospital, and then analyzed the relationship between EGFR mutation status and clinical features. Results Our study showed 33 occurred EGFR mutation in 100 subjects(33%, 33/100), including 70.97% non-smoking female adenocarcinoma patients. EGFR muta-tional sites mainly exist in exon 19 and 21. EGFR mutation is related to pathological patterns, gender, smoking history, but has no relationship to clinical stages. It seems like having some correlation between mutation status and degree of pathological dif-ferentiation. Conclusions Revealing EGFR gene mutation characteristics and relationship with clinical features could help to advise potential EGFR mutation patients who would probably gain benefit from TKI.关键词
肺癌/表皮生长因子受体/突变/焦磷酸测序Key words
Lung cancer/Epidermal Growth Factor Receptor/Mutation/Pyrosequencing分类
医药卫生引用本文复制引用
喻巍,吴智宁,梁剑平,杨德松,王文祥,周勇,李旭,张百华,唐金明,吴劼..连续100例肺癌术后 EGFR 基因突变检测结果分析[J].肿瘤药学,2016,6(2):150-153,4.基金项目
湖南省科技计划项目（2013SK5010）。 （）
