作物研究2016，Vol.30Issue(3)：237-240,4.DOI:10.16848/j.cnki.issn.1001-5280.2016.03.01
拟南芥 CWINV1启动子 GUS 表达载体构建及转基因植株的鉴定
The GUS Expression Vector Construction of Arabidopsis CWINV1 Promoter and Identification of Transgenic Plants
摘要
Abstract
CWINV gene encoding the cell wall invertase.CWINV play an important role in plant growth and development, especially blooming,fruit setting,seed formation and other reproductive development period.But its function has not been e-lucidated in detail.In order to further exploring the mechanism of CWINV gene expression and regulation,the plant expres-sion vectors of CWINV1 gene promoter was constructed with GUS fused report genes,and then transformed into Arabidopsis, transgenic plants were obtained.GUS staining results further verifies the correctness and practicability of the recombinant expression vector.关键词
拟南芥/CWINV1/载体构建/GUS 染色Key words
Arabidopsis/CWINV1/vector construction/GUS staining分类
生物科学引用本文复制引用
程姣,黄弈,任春梅..拟南芥 CWINV1启动子 GUS 表达载体构建及转基因植株的鉴定[J].作物研究,2016,30(3):237-240,4.基金项目
国家自然科学基金项目（30671121）；国家级大学生研究性学习和创新性实验计划项目（（G）SCX1512）。 （）
