作物研究2016，Vol.30Issue(3)：241-246,6.DOI:10.16848/j.cnki.issn.1001-5280.2016.03.02
高羊茅 FaSGR 基因 RNAi 载体构建
Construction of RNAi Expression Vector for FaSGR Gene of Tall Fescue
摘要
Abstract
In this study,RNAi vector pANDA-B-870 with hygromycin resistance for tall fescue FaSGR gene drove by Ubiq-uitin promoter was constructed using the Gateway technology,which was suitable for genetic transformation of monocots.The pANDA-B-870 vector was transformed into Agrobacterium tumefaciens EHA105 competent cells by electroporation method, the transformation was confirmed by PCR.The construction of RNAi vector for FaSGR gene provided technical support for further study of FaSGR gene’s function and the application of SGR of tall fescue in genetic improvement.关键词
高羊茅/FaSGR 基因/载体构建/RNA 干扰/Gateway 技术Key words
tall fescue/FaSGR gene/vector construction/RNA interference/Gateway technology分类
生物科学引用本文复制引用
文昭竹,尚丹,龚梨霞,张志飞..高羊茅 FaSGR 基因 RNAi 载体构建[J].作物研究,2016,30(3):241-246,6.基金项目
中国博士后科学基金项目（2014M560643）；湖南省教育厅优秀青年基金（14B083）；湖南农业大学研究生创新项目。 （）
