安徽医科大学学报2016，Vol.51Issue(4)：484-488,5.
模拟微重力对猕猴肺组织趋化因子CCL20及其受体CCR6表达的影响
Effect of simulated weightlessness on the expression of chemokine CCL20 and it′s receptor CCR6 in lung of rhesus monkey
摘要
Abstract
Objective To investigate the effect of simulated weightlessness on the mRNA and protein expression of chemokine CCL20 and it′s receptor CCR6 in lung of rhesus macaque. Methods Fifteen healthy young male rhesus monkeys were randomly divided into 3 groups:control group, simulated group and recovery group. HE staining was used to observe the histopathological structure changes of pul-monary tissues. And the mRNA and protein expres-sion of CCR6 and CCL20 in lung tissue were detected by immunohistochemistry and quantitative real-time PCR( Q-PCR) . Results Compared with the control group, histopathological examination revealed alveolar septal thicken-ing, and alveolar and interstitial lymphocytic infiltration in simulated group and recovery group, and the pathologi-cal changes in recovery group were lighter than those in simulated group. The expressions of CCL20 mRNA in simu-lated group and the recovery group were higher than that in the control group, but there was no significant differ-ence;the expression of CCR6 mRNA in simulated group was significantly higher than that in the control group and the recovery group(P<0. 01), but there was no significant difference between the control group and the recovery group. Immunohistochemistry results showed that CCL20 and CCR6 were expressed in the lung tissues of each group, but the expression of CCL20 was weak. The positive cells were found mainly in the cytopl-asm of bronchial and alveolar epithelial cells and vascular endothelial cells. The protein expression of CCL20 and CCR6 in simulated group were significantly higher than that in the control group and the recovery group(P<0. 05). Conclusion Me-dium or long term simulated weightlessness can induce the destruction of lung tissue structure and infiltration of lymphocytes, and it can also significantly enhance the mRNA and protein expression of CCL20 and its receptor CCR6 in lung tissues.关键词
模拟微重力/猕猴/肺组织/CCL20/CCR6Key words
simulated weightlessness/rhesus macaque/lung/CCL20/CCR6分类
医药卫生引用本文复制引用
许崇玉,王萍,陈杨,朱敏立,司少艳,蔡艺灵,马华松,陈志明..模拟微重力对猕猴肺组织趋化因子CCL20及其受体CCR6表达的影响[J].安徽医科大学学报,2016,51(4):484-488,5.基金项目
总装备部试验技术研究重点项目(编号:SMFA13K02) （编号:SMFA13K02）
