安徽医科大学学报2016，Vol.51Issue(4)：493-496,4.
化学合成microRNA-29 a inhibitors和mimics对SD大鼠心肌成纤维细胞活化增殖的影响
Effect of microRNA-29 a inhibitors and mimics on proliferation and activation of cardiac fibroblasts in rats
摘要
Abstract
Objective To explore the effection of miR-29 a inhibitors and mimics on proliferation and activation of cardiac fibroblasts in rats. Methods Cardiac fibroblasts were isolated from newly born male SD rats. miR-29a in-hibitors or mimics was transfected respectively into cardiac fibroblasts through LipofectamineTM 2000 Reagent. Twenty-four and Forty-eight hours after transfection, the expressions of miR-29a and mRNA of Col1A1 andα-SMA were analyzed by qRT-PCR. MTT assay was used to detect the proliferation activity of the transfected cardiac fibro-blasts. Results Compared with normal group and negative group, expression of miR-29a was down-regulated in miR-29a inhibitors group while up-regulated in miR-29a mimics group. Col1A1 mRNA expression was up-regulated in miR-29a inhibitors group while down-regulated in miR-29a mimics group. α-SMA mRNA expression was up-reg-ulated in miR-29a inhibitors group while down-regulated in miR-29a mimics group. The cardiac fibroblasts prolifer-ation activity decreased significantly after transfected miR-29a mimics while increased in miR-29a inhibitors groups. Conclusion miR-29a mimics can suppress the proliferation activity of cardiac fibroblasts significantly, implicating miR-29 a as a potential target for cardiac fibroblasts activation and proliferation and pointing to this result can pro-vide new way of thinking of interventions designed to prevent the cardiac fibrosis occurrence and development.关键词
microRNA-29a/心肌成纤维细胞/Col1A1/α-SMA/quantitative real-time PCRKey words
microRNA-29 a/cardiac fibroblasts/Col1 A1/α-SMA/qRT-PCR分类
医药卫生引用本文复制引用
陈泽文,陶辉,周晓,施鹏,张家贵,宣海洋,占红英,石开虎..化学合成microRNA-29 a inhibitors和mimics对SD大鼠心肌成纤维细胞活化增殖的影响[J].安徽医科大学学报,2016,51(4):493-496,4.基金项目
国家自然科学基金资助项目(编号:81570295) （编号:81570295）
安徽省自然基金(编号:1308085MH117、1408085MH175) （编号:1308085MH117、1408085MH175）
