安徽医科大学学报2016，Vol.51Issue(4)：574-577,578,5.
85株儿童感染金黄色葡萄球菌耐药性及流行病学研究
Antimicrobial resistance and epidemiology of 85 isolates of Staphylococcal aureus infection in paediatrics
摘要
Abstract
Objective To investigate the antimicrobial resistance and the epidemiological characteristics of Staphylo-coccus aureus( SA) . To analyze the methicillin-esistant SA( MRSA) staphylococcal cassette chromosome ( SCCmec) genotype distribution characteristics, so as to provide evidence for clinical infection and control. Methods A total of 85 clinical isolates of SA were consecutively collected from different clinical significance specimens, and were ex-cluded the repeated isolates from the same patients. All isolates SA and drug sensitivity test were identified by the Microscan Walk-Away-40 automatic microorganism analyzer. The MRSA was made by agar dilution method for phe-notype screening and further identified by polymerase chain reaction ( PCR) . The genotypes of SCCmec were deter-mined by multiplex PCR. Results Of 85 strains isolated SA, there were 19 strains MRSA and 66 strains methicil-lin sensitive SA ( MSSA) . The detection rate of MRSA was 22. 35%. The main detection rate of MRSA specimens was sputum and throat swab,followed by secretions. All SA were completely resistant to penicillin and childamycin, but entirely sensitive to vancomycin. Compared with MSSA, the drug resistance of 16 common antimicrobial agengts was higher than that of MRSA. SCCmec genotyping results showed that the most frequent SCCmec type wasⅢ. The 12 isolates of MRSA were SCCmecⅢ, only 5 isolates were SCCmecⅠ, and 2 isolates had no genotype. TypeⅡ, type Ⅳ and typeⅤwere not found in the group. Conclusion There are different resistance patterns of SA in the local area. The rates of drug resistance of MRSA for children are higher than those of MSSA. The main SCCmec genotype of MRSA in children is SCCmecⅢ. Clinician should use antimicrobial drugs rationally, and furthermore, we should regularly monitor the change of resistance and prevent nosocomial transmission.关键词
儿童/金黄色葡萄球菌/耐药性/葡萄球菌盒式染色体/聚合酶链反应Key words
children/Staphylococcus aureus/antimicrobial-resistance/staphylococcal cassette chromosome mec/polymerase chain reaction分类
医药卫生引用本文复制引用
夏小荣,熊玉玲,朱玉林,王亚亭..85株儿童感染金黄色葡萄球菌耐药性及流行病学研究[J].安徽医科大学学报,2016,51(4):574-577,578,5.基金项目
安徽省自然科学基金(编号:1408085QH157) （编号:1408085QH157）
