安徽医科大学学报2016，Vol.51Issue(5)：615-619,5.
PICK1对人肝癌细胞HepG2增殖的影响
Function of PICK1 on proliferation of human HepG2 cells
摘要
Abstract
Objective To investigate the probably value of PICK1 in HCC patients after hepatectomy and to deter-mine the effects of PICK1 on the proliferation of human hepatocellular carcinoma cell lines HepG2 . Methods The differential expression of PICK1 in paired tumor and non-tumorous tissue was evaluated by RT-PCR, Western blot and immunohistochemistry. Exposure HepG2 cells with different concentrations of FSC-231(a small molecule inhib-itor of the PICK1 PDZ Domain). MTT assay was used to evaluate the inhibitory rate of proliferation of HepG2. Af-ter 24 h treatment of FSC-231 in HepG2 cells, the modulation of FSC-231 on cell cycle distribution of HepG2 cells was detected by flow cytometer, the CyclinD1, C-myc, Notch1, Hes-1 expression of HepG2 cells were examined by RT-PCR and Western blot. Results Compared with paired non-tumorous tissues, PICK1 were highly expressed in HCC tissue, significantly increased mRNA and protein level of PICK1 was observed in HCC tissue(P<0. 05);FSC-231 was able to effectively inhibit cell proliferation in a concentration and time dependent pattern and signifi-cantly decreased the protein levels of CyclinD1,C-myc and Notch1, Hes-1(P<0. 05). Conclusion PICK1 may be a potential therapeutic target gene for human hepatocellular carcinoma, probably correlating with Notch signal pathway.关键词
PICK1/FSC-231/HepG2 细胞/肝癌/肝细胞增殖/CyclinD1/Notch-1/Hes-1Key words
PICK1/FSC-231/HepG2 cell/HCC/cell proliferation/CyclinD1/Notch1/Hes-1分类
医药卫生引用本文复制引用
谢娟,周群,刘雪姣,黄成,孟晓明,李俊..PICK1对人肝癌细胞HepG2增殖的影响[J].安徽医科大学学报,2016,51(5):615-619,5.基金项目
国家自然科学基金(编号:81473268、81273526) （编号:81473268、81273526）
安徽省科技攻关计划(编号:1301042212) （编号:1301042212）
安徽省自然科学基金项目(编号:1308085MH145) （编号:1308085MH145）
