安徽医科大学学报2016，Vol.51Issue(5)：649-654,655,7.
Kv1．3在LPS诱导的小鼠急性肝损伤模型中表达变化
Expression profile of voltage-gated potassium channel Kv1. 3 in LPS-induced acute liver injury
摘要
Abstract
Objective To investigate the expression profile of voltage-gated potassium channel Kv1. 3 in lipopo-lysaccharide(LPS)-induced acute liver injury. Methods C57BL/6 mice were injected with LPS intraperitoneally for 24 h,to establish the model of acute liver injury. The morphological changes of liver tissues were observed by HE staining. Alanine aminotransferase ( ALT) and aspartate aminotransferase ( AST) levels were measured using test kits. The Kv1. 3 expression was measured by immunohistochemistry, Florescent real-time quantitative RT-PCR ( qRT-PCR) and Western blot. Kupffer cells ( KCs) were isolated by in situ perfusion, and then applied to detect Kv1. 3, tumor necrosis factor alpha(TNF-α) expression by qRT-PCR. The expression of Kv1. 3 was also detected in LPS-stimulated RAW264. 7 cells. Margatoxin( MgTx) was pretreated to block Kv1. 3 in LPS-stimulated RAW264. 7 cells, then detected the expression of cytokines by qRT-PCR and Western blot. Results HE staining showed that liver tissueswere damaged by LPS,ALT and AST levels increased significantly after LPS treatment(P<0. 01). The Kv1. 3 expression decreased significantly in model group compared to normal group(P<0. 01). Kv1. 3 was low ex-pressed in KCs in LPS-inducedacute liver injury model group ( P <0. 01 ) . LPS-stimulated RAW264. 7 cells ex-pressedlow level of Kv1. 3(P<0. 05), and MgTx, the Kv1. 3 specific blocker, decreased LPS-induced cytokynesis secretion. Conclusion The results indicate that Kv1. 3 is low expressed in LPS-induced acute liver injury and bloc-king of the Kv1. 3 channel decreases LPS-induced cytokynesis secretion in macrophage. Kv1. 3 may play an impor-tant role in the treatment of liver diseases.关键词
离子通道/急性肝损伤/细胞因子/枯否细胞/T淋巴细胞Key words
ion channel/acute liver injury/cytokynesis/Kupffer cells/T lymphocyte分类
医药卫生引用本文复制引用
周群,吴宝明,孟晓明,黄成,李俊..Kv1．3在LPS诱导的小鼠急性肝损伤模型中表达变化[J].安徽医科大学学报,2016,51(5):649-654,655,7.基金项目
国家自然科学基金(编号:81273526、81473268、81500473) （编号:81273526、81473268、81500473）
安徽省科技攻关计划项目(编号:1301042212) （编号:1301042212）
安徽省自然科学基金(编号:1308085MH145) （编号:1308085MH145）
