安徽医科大学学报2016，Vol.51Issue(5)：659-663,5.
青春期和成年早期铅、镉联合暴露对小鼠雄性生殖功能的影响
Effects of lead and cadmium co-exposure during puberty and early adulthood on male reproduction in mice
摘要
Abstract
Objective To observe the effects of lead and cadmium co-exposure through drinking water during pu-berty and early adulthood on male reproduction in mice. Methods Healthy male ICR mice at 4 weeks were ran-domly divided into the control, lead, cadmium, lead plus cadmium group. There were 15 mice in each group. Mice in each group were exposed to pure water ( reverse osmosis water) , lead acetate solution, cadmium chloride solution, and the mixed solution including lead acetate and cadmium chloride. After exposure of 40 days, blood was collected by removing eyeballs, and then mice were killed. The testes, epididymides were detached and weigh-ted. The organ coefficient of testes and epididymides was counted. Sperm was counted from cauda epididymidis. Testicular histological morphology was evaluated using H&E staining. Serum testosterone concentration was ana-lyzed using enzyme linked immunosorbent assay ( ELISA) . Results No significant difference in water consump-tion, diet intake, and body weight gain was observed among cadmium, lead, cadmium plus lead co-exposure group and the control group. After exposure of 40 days, lead, cadmium or lead plus cadmium did not cause the change of body weight, the weight of testes and epididymides, and organ coefficient of testes and epididymides. Further re-search showed that no obvious difference in sperm count and serum testosterone concentration was observed among the four groups. Conclusion The present study shows that lead and cadmium co-exposure during puberty and ear-ly adulthood does not affect male reproduction in mice.关键词
镉/铅/小鼠/联合染毒/睾丸/睾酮Key words
lead/cadmium/mouse/combined exposure/testis/testosterone分类
医药卫生引用本文复制引用
张莉,胡仁宜,王华,杨露露,胡永方,王碧伟,张桂彬,徐德祥..青春期和成年早期铅、镉联合暴露对小鼠雄性生殖功能的影响[J].安徽医科大学学报,2016,51(5):659-663,5.基金项目
国家自然科学基金项目(编号:81473016) （编号:81473016）
安徽医科大学中青年学术骨干资助基金 （）
