北华大学学报（自然科学版）2016，Vol.17Issue(3)：315-321,7.DOI:10.11713/j.issn.1009-4822.2016.03.007
俄罗斯蓝靛果忍冬优良无性系微繁技术
Micropropagation of Excellent Clones of Russian Lonicera caerulea
摘要
Abstract
L. caerulea excellent clone ( L. caerulea var. kamtschatika X altaica ) , Rus1 ( L. caerulea var. kamtschatika)and Rus2(L. caerulea var. regeliana X altaica) introduced from Russia as the research objects,with its strong branches on the stem tip and stem segments as explants for tissue culture,a complete micro-propagation protocol is developed for Russia L. caerulea. The results showed that the 3 excellent clones of Russian L. caerulea with MS+1. 0 mg/L 6-BA+0. 2 mg/L IBA as primary radical had better effect,but subculture proliferation medium and rooting medium were large differences,which is suitable for training base of the proliferation of Berel MS+2. 0 mg/L 6-BA+0. 2 mg/L IBA,the proliferation coefficient reached 5. 6,rooting culture medium for WPM+1. 5 mg/L IBA and training base for Rus1 and Rus2 proliferation WPM+0. 5 mg/L 6-BA+0. 2 mg/L IBA. The proliferation coefficient reached 6. 8 and 7. 6, and rooting medium for 1/2MS+0. 2 mg/L IBA, Russia L. caerulea test tube seedling transplanting matrix is mixed matrix of turfy soil +sand(rate of volume 1 ∶1).关键词
蓝靛果忍冬/组织培养/无性系Key words
Lonicera edulis/tissue culture/clones分类
农业科技引用本文复制引用
葛丽丽,张启昌,梁天,陈振..俄罗斯蓝靛果忍冬优良无性系微繁技术[J].北华大学学报（自然科学版）,2016,17(3):315-321,7.基金项目
吉林省科技发展计划项目(20120269 （）
20140307025NY) （）
中央财政林业科技推广示范项目(2013TJQ04) （2013TJQ04）
大学生创新项目(201410201076) （201410201076）
