北华大学学报（自然科学版）2016，Vol.17Issue(3)：339-343,5.DOI:10.11713/j.issn.1009-4822.2016.03.012
川芎生物碱对大鼠脑缺血-再灌注损伤的保护作用
Protective Effect of Chuanxiong Alkaloid on Cerebral Ischemia Reperfusion Injury in Rats
摘要
Abstract
Objective To explore the protective effects of Chuanxiong ( CHX ) Alkaloids on cerebral ischemia-reperfusion( I/R) injury in rats. Methods A total of 60 wistar rats were purchased and randomly divided into six groups with 10 rats in each grouop, including ischemia-reperfusion ( I/R ) group, sham operated group, the treatment groups and the positive control group. The reversible middle cerebral artery ischemia-reperfusion( I/R) model was made by suture method. Superoxide dismutase( SOD) activity and malondialdehyde( MDA) content of the brain tissue were measured with xanthine oxidase method and thiobarbituric acid method. Neurological injury scores were recorded after the animals regained consciousness,the changes of water content in cerebral tissue was detected,and the results were compared with the sham group. Results Compared with the sham group,in the I/R group,the SOD activity decreased and MDA content increased in brain tissue(P<0. 01),the volume and area of brain infarction significantly increased ( P<0 . 01 ) , the content of brain water increased and neurological seriously damaged(P<0. 05). Compared with I/R group,the SOD activity of CHX Alkaloids treatment groups was significantly higher(P<0. 01),MDA obviously decreased in brain tissue(P<0. 05),water content in brain tissue decreased,the area and volume of infarction significantly reduced(P<0. 01),and neurologic injury score was obviously different(P<0. 01). Conclusion CHX alkaloids can reduce cerebral I/R-induced brain damage,and thus has protective effect on cerebral tissue to some extent.关键词
川芎生物碱/超氧化物歧化酶/丙二醛/脑梗死/缺血再灌注Key words
Chuanxiong Alkaloids/superoxide dismutase/malondialdehyde/cerebral infarction/ischemia-reperfusion分类
医药卫生引用本文复制引用
刘絮,姜秀文,王立英,申旭鹏..川芎生物碱对大鼠脑缺血-再灌注损伤的保护作用[J].北华大学学报（自然科学版）,2016,17(3):339-343,5.基金项目
吉林省科技发展计划项目(20030908-4) （20030908-4）
吉林市科技发展计划项目(201536058) （201536058）
