北华大学学报（自然科学版）2016，Vol.17Issue(3)：352-354,3.DOI:10.11713/j.issn.1009-4822.2016.03.015
左乙拉西坦与苯妥英钠协同抗小鼠最大电惊厥作用实验研究
Experimental Research on the Effects of Levetiracetam Combined with Phenytoin Sodium on Anti-maximal Electric Seizure in Mice
摘要
Abstract
Objective To investigate the synergistic effect of levetiracetam combined with phenytoin sodium on anti-maximal electric seizure ( MES ) in mice. Methods A total of 100 male and 100 female mouse were randomly divided into the normal control group,high dose levetiracetam group,middle dose levetiracetam group, low dose levetiracetam group,high dose phenytoin sodium group,middle dose phenytoin sodium group and low dose phenytoin sodium group. Dose-effect relationships of anti-MES were measured. According to the dose-effect relationships,the corresponding anti-MES’ s effect drug doses were determined. They were high dose group, middle dose group and low dose group with 20 mouse in each group. The control group was given 10 mL/kg saline by gavage,and the other three groups were administrated the corresponding drugs. After 50 minutes,all the mice were treated with electricity. When the lower limbs and the whole body of the mice showed tonic convulsions,the convulsion frequencies were recorded and then the convulsion rates were calculated. Results Both levetiracetam and phenytoin sodium had good anti-electric convulsion effect and showed obvious dose-effect relationship. The combination use of subthreshold doses of levetiracetam and phenytoin sodium could produce synergistic anti-maximal convusion effect,with convulsion rate of 10% in high dose group and the rate of 40% in middle dose group. Conclusion The subthreshold dose of levetiracetam and phenytoin has obvious synergistic anti-MES effect and shows a good dose-effect relationship.关键词
电惊厥/左乙拉西坦/苯妥英钠/协同作用Key words
MES/levetiracetam/phenytoin sodium/synergistic effect分类
医药卫生引用本文复制引用
李冰,齐春丽..左乙拉西坦与苯妥英钠协同抗小鼠最大电惊厥作用实验研究[J].北华大学学报（自然科学版）,2016,17(3):352-354,3.基金项目
吉林省科技发展计划项目(20130633) （20130633）
