重庆医学2016，Vol.45Issue(10)：1302-1305,4.DOI:10.3969/j.issn.1671-8348.2016.10.002
重组人乳铁蛋白对幽门螺杆菌抑菌作用的实验研究
Experimental study on bacteriostatic effect of recombinant human lactoferrin on Helicobacter pylori
摘要
Abstract
Objective To evaluate the bacteriostatic effect of recombinant human lactoferrin(rhLF) on Helicobacter(H .) py‐lori and its influence on CagA ,Ure and gastric mucosal IL‐8 .Methods The minimum inhibitory concentration(MIC)and the influ‐ence of different drug concentrations on the proliferation of H .pylori were detected .The effects of rhLF on the mRNA and protein expressions of CagA and Ure in H .pylori were detected by RT‐PCR and Western blot ,respectively .The animal study :Balb/c mice were adopted and assigned randomly into four groups ,including the standard triple+rhLF(group A) ,rhLF(group B) ,standard tri‐ple(group C) and normal saline(group D) .The histopathological HE staining was used to observe the gastric inflammation and ELISA was used to detect the IL‐8 level of gastric tissue in each group .Results MIC was 0 .5 mg/mL ,moreover rhLF inhibited the bacterial growth and proliferation with a concentration‐dependent manner .rhLF could reduce the expression of H .pylori major viru‐lence factor CagA ,mRNA and protein of Ure .Comparing the group A with the group B ,C and D ,the gastric mucosal inflammation score and the IL‐8 levels of gastric tissue homogenates had statistically significant differences(P<0 .05) .Conclusion rhLF inhibits the growth and proliferation of H .pylori ,moreover inhibit the expression of major virulence factor CagA in H .pylori ,mRNA and protein of Ure in different degrees ,weakens its pathogenicity ,meanwhile reduces the IL‐8 level in mice gastric mucosa ,and allevi‐ates H .pylori related gastric mucosal inflammatory response .关键词
螺杆菌 ,幽门/乳铁蛋白/细胞毒素相关蛋白A/尿素酶/白细胞介素8Key words
helicobacter pylori/lactoferrin/cytotoxin associated gene A/urease/interleukin-8分类
医药卫生引用本文复制引用
罗娟,董向前,杨刚,南琼,马岚青,成国祥,袁渝萍,张爱民,刘雪芳,刘思国,边莉,陈建泉,张磊..重组人乳铁蛋白对幽门螺杆菌抑菌作用的实验研究[J].重庆医学,2016,45(10):1302-1305,4.基金项目
云南省联合专项基金资助项目（2012FB027）。 （）
