重庆医学2016，Vol.45Issue(11)：1444-1447,4.DOI:10.3969/j.issn.1671-8348.2016.11.002
FOXC2对结直肠癌细胞上皮-间质转化及侵袭能力的影响
Effect of FOXC2 on epithelial-mesenchymal transition and invasion of colorectal cancer cells
摘要
Abstract
Objective To identify the role of FOXC2 in the invasion and migration of colorectal cancer cells .Methods Stable cell lines expressing FOXC2(SW480/FOXC2) or vector (SW480/pBabe) were established using retroviral infection method .The morphology alterations of SW480 cells were observed using a microscope .Western blot analysis and immunofluorescence staining assays were performed to detect the expression of E‐cadherin ,Vimentin and N‐cadherin .The invasive and migratory abilities of colorectal cancer cells evaluated using Transwell invasion chamber experiment detection .Results The morphology of SW480 cells was significantly changed after overexpression .From the original shape typical of epithelial cells became spindle shaped growth , similar to the morphology of fibroblasts .Western blot analysis and immunofluorescence staining displayed that overexpression of FOXC2 led to significant downregulation of the epithelial marker E‐cadherin ,but upregulation of the mesenchymal markers Vimen‐tin and N‐cadherin .Transwell assay reveals that overexpression of FOXC2 strongly enhanced the migratory and invasive ability of SW480 cells .Conclusion FOXC2 induces epithelial‐mesenchymal transition and promotes the invasive ability of colorectal cancer cells .关键词
结直肠肿瘤/叉头框C2/上皮-间质转化/侵袭Key words
colorectal neoplasms/FOXC2/epithelial-mesenchymal transition/invasion分类
医药卫生引用本文复制引用
蔡娟娟,崔艳梅,丁彦青,廖雯婷..FOXC2对结直肠癌细胞上皮-间质转化及侵袭能力的影响[J].重庆医学,2016,45(11):1444-1447,4.基金项目
国家自然科学基金资助项目（U1201226，81472710，81172055）；广州市珠江科技新星专项项目（2012J2200052，2012J2200044）。 （）
