重庆医学2016，Vol.45Issue(11)：1473-1476,4.DOI:10.3969/j.issn.1671-8348.2016.11.010
PPA Rγ激动剂干预对急性胰腺炎小鼠肝损伤的影响
The effect of PPARγ agonists in acute pancreatitis with hepatic injury
摘要
Abstract
Objective To observe the peroxidase body growth activated receptor γ (PPARγ) agonist rosiglitazone on acute pancreatitis in mice with hepatic injury and to investigate the mechanism of hepatic injury .Methods Seventy‐two male Kunming mice were randomly allocated into three groups(24 cases for each group):acute pancreatitis group(AP group) ,rosiglitazone group (AP‐ROS group) ,saline group(NS group) .Mice were killed at 6 ,12 and 24 h after induction of acute pancreatitis .Serum amylase , ALT and AST activities were measured .The expressions of NF‐κB and PPARγmRNA were assessed by RT‐PCR .The expressions of NF‐κB and PPARγ protein were assessed by Western blot .Results Compared with NS group ,serum amylase ,ALT and AST levels at each time point significantly increased in AP group(P< 0 .01);serum amylase ,ALT and AST levels in AP‐ROS group were significantly lower than those in AP group(P<0 .01) .Compared with NS group ,the expressions of liver PPARγ mRNA and protein in AP group were markedly lower at 6 h and 12 h(P<0 .05) ,and the expressions of PPARγmRNA and protein in AP‐ROS group were significantly higher than those in NS group and AP group(P<0 .01) .The expressions of liver NF‐κB mRNA and NF‐κB p65 protein in AP group were significantly higher than those in NS group and AP‐ROS group at all time points(P<0 .01) .Con‐clusion There are clear relationships between NF‐κB and hepatic injury in acute pancreatitis .The expressions of PPARγin injuried hepatic decreased .Rosiglitazone can increase the expressions of PPARγand prevent the expressions of NF‐κB in hepatic during the early phase of acute pancreatitis .关键词
核因子-κB/急性胰腺炎/肝损伤/雨蛙肽/过氧化物酶体增殖物激活受体γKey words
NF-κB/acute pancreatitis/hepatic injury/cerulein/peroxidase body growth activated receptor γ分类
医药卫生引用本文复制引用
马增翼,许刚,于文光,田克立..PPA Rγ激动剂干预对急性胰腺炎小鼠肝损伤的影响[J].重庆医学,2016,45(11):1473-1476,4.基金项目
山东省科技发展计划（2006GG2202021）；山东省自然科学基金（Y2005C29）。 （）
