重庆医学2016，Vol.45Issue(11)：1484-1487,4.DOI:10.3969/j.issn.1671-8348.2016.11.013
SpA 刺激单核巨噬细胞表达早期致炎因子的实验研究
Effect of staphylococcal protein A on pro-inflammatory cytokines expression in mononuclear macrophage
摘要
Abstract
Objective To study the expression and regulation of pro‐inflammatory cytokinesTNF‐α,IL‐1 ,IL‐6 in mononucle‐ar macrophages stimulated with staphylococcal protein A (SpA) .Methods THP‐1 was incubated with PMA and induced into mononuclear macrophages .Then the macrophages were incubated with varying concentrations of SpA under different time points . The effect of SpA on macrophage proliferation was measured by MTT method .The levels of inflammatory cytokines ,TNF‐α,IL‐1 and IL‐6 from the cultured cell media were measured by ELISA respectively .The levels of mRNA expression corresponding to TNF‐α,IL‐1 and IL‐6 were detected by RT‐PCR from the macrophages stimulated with SpA .All statistical analyses were performed by SPSS17 .0 software .Results The MTT result indicated that SpA had a positive effect on the proliferation of THP‐1 cells in a dosage depended manner .The addition of SpA could enhance the mRNA expression of TNF‐α,IL‐1 and IL‐6 in the stimulated mac‐rophages .It also showed a specific dose‐effect and time‐effect correlation .The macrophages secreted inflammatory cytokines and its corresponding mRNA reached its peak levels at 12 h post stimulation .Compared with the control group ,the expression and release of TNF‐α,IL‐1 and IL‐6 in macrophages from the experimental group was increased with statistical significance(P<0 .01) .Conclu‐sion SpA can promote the secretion and expression of early pro‐inflammatory cytokines ,such as TNF‐α,IL‐1 and IL‐6 in macro‐phages .Therefore ,SpA plays a very important role in the initiation and development of the staphylococcus aureus sepsis .关键词
金黄色葡萄球菌蛋白A/T HP-1/肿瘤坏死因子/白细胞介素-1/白细胞介素-6Key words
staphylococcal protein A/T HP-1/tumor necrosis factor-alpha/interleukin-1/interleukin-6分类
医药卫生引用本文复制引用
马兴龙,黄俞霖,陈晓晴,李明强,刘清蒙..SpA 刺激单核巨噬细胞表达早期致炎因子的实验研究[J].重庆医学,2016,45(11):1484-1487,4.基金项目
贵州省大学生创新课题（201413653004）。 （）
