重庆医学2016，Vol.45Issue(11)：1515-1518,4.DOI:10.3969/j.issn.1671-8348.2016.11.022
miRN A-125b在鼻咽癌中的表达及与顺铂化疗敏感性研究
Study on expression of miRNA-125b in nasopharyngeal carcinoma and sensitivity of cisplatin chemotherapy
龚轩民 1孙永东2
作者信息
- 1. 四川医科大学中西医结合学院，四川泸州646000
- 2. 四川医科大学附属中医医院耳鼻喉科，四川泸州646000
- 折叠
摘要
Abstract
Objective To investigate the whether miR‐125b expression having the abnormality in nasopharyngeal carcinoma tissues and serum and whether having the correlation with the sensitivity of cisplatin chemotherapy .Methods The intact medical records and tissue and serum samples in 34 cases of clinically diagnosed nasopharyngeal carcinoma were collected and the serum samples in 34 individuals undergoing the physical examination were also collected as the controls .The expression of miRNA‐125b in carcinoma tissue ,paracancerous tissue and serum was detected by real‐time quantitative polymerase chain reaction (real‐time qPCR) .The statistical analysis was performed .Results The expression of miRNA‐125b in carcinoma tissue was significantly lower than that in paracancerous tissue(P=0 .006) ,and serum miRNA‐125b expression in nasopharyngeal carcinoma patients was signifi‐cantly lower than that in the individuals undergoing the healthy physical examination(P=0 .000);but the miRNA‐125b expression had no correlation between the paracancerous tissue and serum(r=0 .112 ,P=0 .528) .The expression of miRNA‐125b in cancer tis‐sues was correlated with the pathological typing ,T staging and N staging(P<0 .05) ,the serum miRNA‐125b expression in carcino‐ma subjects was only related with the N staging (P<0 .05) .The expression of miRNA‐125b in carcinoma tissue was negatively cor‐related with the sensitivity of cisplatin chemotherapy (P< 0 .05) ,however ,there was no obvious correlation between the serum miRNA‐125b level and the sensitivity of cisplatin chemotherapy (P>0 .05) .Conclusion The low miRNA‐125b expression may be involved in the occurrence and development of nasopharyngeal carcinoma and plays a certain significance for the diagnosis ,pathology typing and classification of nasopharyngeal carcinoma .Its expression level in cancer tissue can serve as one of indicators for screen‐ing out the cisplatin chemotherapy sche.关键词
微RNA-125b/鼻咽肿瘤/顺铂/化学疗法/敏感性Key words
microRNA-125b/nasopharyngeal neoplasms/cisplatin/chemotherapy/sensitivity分类
医药卫生引用本文复制引用
龚轩民,孙永东..miRN A-125b在鼻咽癌中的表达及与顺铂化疗敏感性研究[J].重庆医学,2016,45(11):1515-1518,4.
