重庆医学2016，Vol.45Issue(12)：1595-1597,3.DOI:10.3969/j.issn.1671-8348.2016.12.004
基因芯片筛选稳定转染FATE／BJ-HCC-2基因的肝癌细胞中肿瘤转移相关差异表达基因
Screening of tumor metastasis related differentially expressed genes in hepatocellular carcinoma cells with FATE/BJ-HCC-2 gene stable transfection by gene chip
摘要
Abstract
Objective To screen the tumor metastasis related differentially expressed genes in hepatocelluar carcinoma (HCC)cells 7402 after stable transfection with FATE/BJ‐HCC‐2 gene .Methods Total RNA was extracted from FATE/BJ–HCC‐2‐transfected HCC(5B4)cells and empty vector control (Mock)cells respectively .Differentially expressed genes were obtained using cDNA microarray .Results Compared with Mock cells ,a total of 1 694 differentially expressed genes were screened out in 5B4 cells ,the 11 gene expressions had obvious differences ,among which the expression amounts in 7 genes were significantly in‐creased ,including MMP‐1 ,PTGS2 ,FN ,CA9 ,IL‐8 ,ILK and Areg .The fold changes were 81 .80 ,49 .86 ,11 .30 ,16 .26 ,3 .48 ,2 .79 and 2 .20 ,respectively .The expression amounts in 4 genes were significantly decreased ,including E‐cadherin ,E‐cadherin , RHOBTB3 ,ALPP and HLA‐DRB4 .The fold changes were -5 .42 ,-2 .23 ,-5 .93 and -8 .03 ,respectively .Conclusion Adopting gene microarray technology can carefully screen the differentially expressed genes of FATE/BJ‐HCC‐2 involved HCC metastasis ,its final goal is to lay a solid theoretical foundation for studying the HCC metastasis mechanism .关键词
肝肿瘤/基因芯片/基因差异表达Key words
hepatocelluar neoplasms/gene chip/differential gene expression分类
医药卫生引用本文复制引用
葛丽丽,杨小昂,朴军颜,尹艳慧,张毓..基因芯片筛选稳定转染FATE／BJ-HCC-2基因的肝癌细胞中肿瘤转移相关差异表达基因[J].重庆医学,2016,45(12):1595-1597,3.基金项目
国家自然科学基金资助项目（81071724）；河南省基础与前沿基金资助项目（142300413224）。 （）
