吉林医药学院学报2016，Vol.37Issue(3)：161-166,6.
功能性支链的合成及靶向性CPPs修饰纳米脂质体的初步理化性质评价
The study on synthesis process of functional branch and the preliminary e-valuation on physicochemical properties of targeting CPPs-modified nanoli-posomes
摘要
Abstract
Objective To synthesize the functional branch and prepare the targeting CPPs-modified liposomes which physicochemical properties are also characterized preliminary in vitro.Methods Polypeptides-modified PEG-DSPE functional branch was synthesized by specific reaction between maleimide and thiol group.Thin-membrane dispersed method was applied to construct targeting CPPs-modified nanoliposomes.In vitro physicochemical properties including mean particles size,surface potential,encapsulation efficiency,polydispersity indexs and in vitro release were studied by laser particle size analyzer and ultraviolet fluorescence spectrophotometry.Results Under the condition of deaer-ation by nitrogen stripping,functional branch could be successful synthesized through Michael addition reaction.Targe-ting CPPs-modified nanoliposomes may be prepared favorably by ammonium sulfate gradient strategy and their physico-chemical properties in vitro were studied.Conclusion This research provide valuable reference for the synthesis strategy applied in functional branch of liposomes and give an early foundation for next biological properties research.关键词
功能性支链/合成/Michael加成反应/纳米脂质体Key words
functional branch/synthesis/michael addition reaction/nanoliposomes分类
医药卫生引用本文复制引用
时念秋,郝乘仪,冯波,张秀荣,李正强,齐宪荣..功能性支链的合成及靶向性CPPs修饰纳米脂质体的初步理化性质评价[J].吉林医药学院学报,2016,37(3):161-166,6.基金项目
吉林市科技发展计划资助项目201464053）；中国博士后科学基金面上项目2015 M571374）；吉林省科技发展计划项目20140311110YY ）；吉林省教育厅资助项目2015401）． （）
