吉林医药学院学报2016，Vol.37Issue(3)：184-187,4.
pIRES2－MLAA34－EGFP穿梭载体的构建及表达
Construction of pIRES2-MLAA34-EGFP vector and its expression
摘要
Abstract
Objective To construct the pIRES2-MLAA34-EGFP recombinant vector and express the MLAA34-EGFP recombinant proteins in E.coli.Methods The MLAA34 gene were extracted from U937 cells by RT-PCR and insert into the pIRES2-EGFP vector.Results We amplified full-length sequence of MLAA-34 gene successfully and iden-tified the correctness of pIRES2-MLAA34-EGFP recombinant vector by PCR and BamHⅠ/EcoRⅠdouble digestion. Moreover,the MLAA34-EGFP recombinant proteins expressed in E.coli were consistent with the expected protein. Conclusion We construct the pIRES2-MLAA34-EGFP recombinant vector successfully and the MLAA34-EGFP re-combinant proteins was successfully induced to express by IPTG.关键词
急性白血病/MLAA-34/免疫治疗/核酸疫苗Key words
acute leukemia/MLAA-34/immunotherapy/DNA vaccine分类
医药卫生引用本文复制引用
赵天宇,赵佳琪,范业宁,马春平,张东红,吕扬,赵臣..pIRES2－MLAA34－EGFP穿梭载体的构建及表达[J].吉林医药学院学报,2016,37(3):184-187,4.基金项目
吉林省科技厅自然科学基金项目20160101179JC）；吉林省卫生和计划生育委员会项目2015 Z071）；吉林省大学生创新创业课题2015024）． （）
