吉林医药学院学报2016，Vol.37Issue(3)：191-193,3.
青蒿素对人肝癌HepG2细胞增殖的影响
The proliferation effect of artemisinin on human hepatoma HepG2 cells
摘要
Abstract
Objective To explore the effects of artemisinin with various concentrations and at different time points on human hepatocellular carcinoma cells HepG2 ) .Methods HepG2 cells in logarithmic growth phase were seeded into 96-well plates and treated by artemisinin 0.1,0.2,0.4,0.6,0.8,1.0,1.2 mmol/L).Then the cell morphology changes were observed under the inverted microscope and the toxicity were detected by MTT measurement after 24 h. Results Under the inverted microscope,with the concentration increased and time went on,the toxic effects on cells can be enhanced,and the number of cells was reduced,most cell volume become shrinkage and small,losing their shape and getting slight rounded.MTT data showed that the artemisinin in different concentrations inhibited HepG2 proliferation in a dose-and time-dependent manner.The concentration of ART was 1.2 mmol/L,the inhibition rates reached 81.33%.Conclusion Artemisinin have a significant inhibition on HepG2 cells proliferation in vitro in a dose-and time-dependent manner.关键词
青蒿素/增殖/人肝癌细胞Key words
artemisinin/proliferation/human hepatocellular carcinoma cell分类
医药卫生引用本文复制引用
岳小庆,徐春芳,胡小龙,谢丹,杨丹,朱文赫..青蒿素对人肝癌HepG2细胞增殖的影响[J].吉林医药学院学报,2016,37(3):191-193,3.基金项目
吉林省2014年大学生创新创业训练计划项目． （）
