南方医科大学学报2016，Vol.36Issue(5)：595-603,9.
兔玻璃体腔注射PBS后视网膜差异蛋白的表达
Proteomic analysis of retinal proteins in rabbits following intravitreal PBS injection：analysis with tandem mass tag labeling coupled with LC-MS/MS
摘要
Abstract
Objective Intravitreal (IVT) injection has become one of the most commonly performed ophthalmologic procedures. We investigated the changes in retinal function and proteomics in rabbits receiving IVT injection of PBS to evaluate the safety of IVT injection. Methods Twenty Chinchilla rabbits were subjected to IVT injection of 50μL PBS in the right eyes. On days 0, 4, 7 and day 14, the retinas of the rabbits were isolated after routine ophthalmic and electroretinogram examinations. The protein expressions in the retinas were quantified using tandem mass tag (TMT)-labeling coupled with LC-MS/MS, and bioinformatic analysis of the differentially expressed proteins (DEPs) was performed based on KEGG database to identify significantly enriched pathways. Functional network of the significant DEPs was analyzed using STRING. Results No noticeable fundus or functional changes occurred in the rabbit eyes following IVT injection of PBS. A total of 6042 retinal proteins were identified in the retina following the injection, among which 49 proteins (0.81%) exhibited over 5.0-fold up-regulation or over 80% down-regulation relative to the control. Most of the distinctly up-regulated or down-regulated proteins were associated with the cytoskeleton. Significantly enriched pathways involved focal adhesion, tight junction, riboflavin metabolism, extracellular matrix-receptor interaction and regulation of actin cytoskeleton. Functional network analysis showed that ACTC1 and ISG15 played central roles in the protein interaction networks. Conclusion IVT PBS injection in rabbits causes alterations in proteins associated with cell adhesion, morphology, migration, differentiation, signal transduction and riboflavin metabolism, but the alterations of the retinal proteins appear not sufficient to cause observable pathology of the retina.关键词
玻璃体腔注射/视网膜/蛋白质组/串联质谱标签Key words
intravitreal injection/retina/proteomics/tandem mass tags引用本文复制引用
汪家名,雷博..兔玻璃体腔注射PBS后视网膜差异蛋白的表达[J].南方医科大学学报,2016,36(5):595-603,9.基金项目
国家自然科学基金（81470621）Supported by National Natural Science Foundation of China (81470621) （81470621）
