南方医科大学学报2016，Vol.36Issue(5)：604-608,5.
茶多酚改善大鼠缺血/再灌注心脏功能和能量代谢并抑制体外培养心肌细胞的钙内流
Tea polyphenols promote cardiac function and energy metabolism in ex vivo rat heart with ischemic/reperfusion injury and inhibit calcium inward current in cultured rat cardiac myocytes
摘要
Abstract
Objective To investigate the protective effects of tea polyphenols (TP) against myocardial ischemia/reperfusion (IR) injuries and explore the possible mechanisms. Methods Langendorff-perfused rat hearts were subjected to ischemia for 30 min followed by reperfusion for another 30 min. Myocardial function indices were measured by a left ventricular cannula via a pressure transducer connected to the polygraph in isolated Langendorff hearts and energy metabolism was measured using 31P nuclear magnetic resonance (NMR) spectroscopy. Whole-cell patch-clamp technique was used to record calcium inward current (ICa- L) in cultured rat cardiac myocytes. Results Compared with the control hearts, the ex vivo rat hearts with 2.5 mg/L TP treatment showed significantly increased left ventricular developed pressure (LVDP), maximal rise rate of LVDP (+dp/dtmax), maximal fall rate of LVDP (-dp/dtmax), and coronary flow (CF) (P<0.05). During both cardiac ischemia and reperfusion phase, ATP and PCr levels were elevated significantly in TP-treated hearts compared with those in the control hearts (P<0.05). In cultured rat cardiac myocytes, ICa-L was remarkably decreased by TP at the doses of 2.5 and 5.0 mg/L (P<0.01). Conclusion Our results support a possible protective role of TP against myocardial IR injury by improving myocardial energy metabolism and inhibiting ICa-L in the cardiac myocytes.关键词
茶多酚/心脏功能/缺血/再灌注/能量代谢/钙内流Key words
tea polyphenols/cardiac function/ischemia/reperfusion/energy metabolism/calcium inward current引用本文复制引用
董华进,李捷,詹皓,李泱,苏瑞斌..茶多酚改善大鼠缺血/再灌注心脏功能和能量代谢并抑制体外培养心肌细胞的钙内流[J].南方医科大学学报,2016,36(5):604-608,5.基金项目
军队医药卫生科研基金（07MB051）Supported by Medical Research Foundation of PLA (07MB051) （07MB051）
