南方医科大学学报2016，Vol.36Issue(5)：639-644,6.
Nanog通过升高PKCɛ的表达促进乳腺癌细胞的侵袭
Nanog promotes the invasion of breast cancer cells by increasing PKCɛexpression
摘要
Abstract
Objective To study the relationship between Nanog- promoted metastasis of breast cancer and ezrinT567 phosphorylation, and explore the possible mechanism by which Nanog regulates ezrinT567 phosphorylation. Methods A siRNA construct targeting Nanog was transfected in breast cancer cells to knock down Nanog expression, and the changes in the cell invasion was detected using Transwell assay. The expression levels of Nanog and PKC and the phosphorylation level of ezrinT567 were detected using Western blotting and immunofluorescent staining; the protein interaction between PKCɛ and ezrin was assayed by co-immunoprecipitation and Western blotting. Results Nanog knockdown significantly decreased the expression of PKCɛprotein, phosphorylation level of ezrinT567 and the invasion ability of breast cancer cells. PKCεknockdown obviously decreased the phosphorylation level of ezrinT567 in the cells, and PKCɛ and ezrin were co-immunoprecipitated. Concludions Nanogcan can upregulate the expression of PKCɛ to promote the phosphorylation of ezrinT567, which can be a new mechanism by which Nanog promotes tumor metastasis.关键词
Nanog/埃兹蛋白/蛋白激酶C/乳腺癌/侵袭Key words
Nanog/ezrin/protein kinase C/breast cancer/invasion引用本文复制引用
邵文凤,李福喜,唐芮,于晓然,温桥生,余焱林,熊静波..Nanog通过升高PKCɛ的表达促进乳腺癌细胞的侵袭[J].南方医科大学学报,2016,36(5):639-644,6.基金项目
国家自然科学基金（81071810，81172058） Supported by National Natural Science Foundation of China (81071810,81172058) （81071810,81172058）
